The feline endogenous RD114 glycoprotein has proved to be an attractive envelope to pseudotype both retroviral and lentiviral vectors. As a surface protein, its detection on packaging cells as well as viral particles would be useful in different fields of its use. To address this, we generated a monoclonal antibody against RD114 by immunization of rats, termed 22F10. Once seroconversion was confirmed, purified 22F10 was cloned into murine Fc and characterized with a binding affinity of 10nM. The antibody was used to detect RD114 and its variant envelopes on different stable viral packaging cell lines (FLYRD18 and WinPac-RD). 22F10 was also shown to prevent the infections of different strains of RD-pseudotyped vectors but not related envelope glycoproteins by blocking cell surface receptor binding. We are the first to report the neutralization of viral particles by a monoclonal αRD114 antibody.

中文翻译：

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针对RD114假型病毒载体的中和抗体的产生。

猫内源性RD114糖蛋白已被证明是逆转录病毒和慢病毒载体假型的诱人包膜。作为一种表面蛋白，它在包装细胞以及病毒颗粒上的检测将在其不同的应用领域中发挥作用。为了解决这个问题，我们通过免疫大鼠产生了一种抗RD114的单克隆抗体，称为22F10。一旦确认了血清转化，就将纯化的22F10克隆到鼠Fc中，并以10nM的结合亲和力表征。该抗体用于检测RD114及其在不同的稳定病毒包装细胞系（FLYRD18和WinPac-RD）上的变异包膜。还显示22F10通过阻止细胞表面受体结合来预防不同菌株的RD假型载体感染，但不能预防相关的包膜糖蛋白感染。