A halophilic bacterial strain, EG1HP4QL, was isolated from a salt sample from Lake Qarun, Fayoum Province, Egypt. Morphological, physiological, biochemical, and phylogenetic analyses indicated that the strain belonged to the genus Halobacillus. Strain EG1HP4QL produced an extracellular polysaccharide (EPS), with production peaking (5.9 g L⁻¹) during growth on medium S-G containing 2% (w/v) sucrose at 35 °C (pH 8.0). The EPS had significant emulsifying activity (E₂₄ %) against kerosene (65.7 ± 0.8%), o-xylene (64.0 ± 1%), and sunflower oil (44.7 ± 0.5%). The composition of the EPS included two polymers—a negatively charged and a neutral one (~ 3:1)—in which mannose and glucose were the main neutral monosaccharide constituents. Strain EG1HP4QL was able to utilize crude oil (35.3%) as the sole carbon source within 12 days. The minimum inhibitory concentrations of heavy metals [Zn(II), Cd(II), Pb(II), Ni(II), and Cu(II)] for strain EG1HP4QL were 1.0, 2.0, 2.0, 2.5, and 5 mM, respectively.

中文翻译：

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嗜盐Halobacillus sp。的生物修复潜力。菌株EG1HP4QL：胞外多糖的产生，原油降解和重金属耐受性。

从埃及法尤姆省卡伦湖的盐样品中分离出嗜盐细菌菌株EG1HP4QL。形态，生理，生化和系统发育分析表明该菌株属于嗜盐杆菌属。菌株EG1HP4QL产生细胞外多糖（EPS），在含2％（w / v）蔗糖的SG培养基上于35°C（pH 8.0）生长期间，产量达到峰值（5.9 g L ^-1）。EPS对煤油具有显着的乳化活性（E ₂₄％）（65.7±0.8％），o-二甲苯（64.0±1％）和葵花籽油（44.7±0.5％）。EPS的组成包括两种聚合物-带负电荷的聚合物和中性的一种聚合物（〜3：1），其中甘露糖和葡萄糖是主要的中性单糖成分。菌株EG1HP4QL能够在12天之内利用原油（35.3％）作为唯一碳源。重金属[Zn（II），Cd（II），Pb（II），Ni（II）和Cu（II）]对EG1HP4QL菌株的最低抑制浓度为1.0、2.0、2.0、2.5和5 mM，分别。