Fruit allergies have become more common in recent years, and are now a serious health problem. In this study, a multiplex PCR assay was used to detect potential fruit allergens causing food allergy labeling in Korea. For the detection of these allergens, specific primer pairs were designed to amplify the allergen-coding genes Cyclophilin (tomato), Mdtl 1 (apple), Pru p 2.01A (peach) and Pectin methylesterase inhibitor (kiwi), and primer pair targeting the 18S ribosomal RNA gene was additionally used as an endogenous control. Primer specificity was assessed with 23 plant species. A mixture of DNA from the four fruits was serially diluted and used to determine the sensitivity of the multiplex PCR assay, which was approximately 0.08 ng. Eleven commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. This assay is expected to be a specific and efficient method for detecting fruit allergens in foods.

中文翻译：

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通过多重 PCR 同时检测番茄、苹果、桃子和猕猴桃中的水果过敏原编码基因。

近年来，水果过敏变得越来越普遍，现已成为一个严重的健康问题。在这项研究中，使用多重 PCR 检测来检测在韩国引起食物过敏标签的潜在水果过敏原。为了检测这些过敏原，设计了特异性引物对来扩增过敏原编码基因亲环蛋白（番茄）、Mdtl 1（苹果）、Pru p 2.01A（桃）和果胶甲酯酶抑制剂（猕猴桃），并设计了针对这些过敏原的引物对。另外使用 18S 核糖体 RNA 基因作为内源对照。对 23 种植物物种的引物特异性进行了评估。来自四种水果的 DNA 混合物被连续稀释并用于确定多重 PCR 检测的灵敏度，约为 0.08 ng。对 11 种商业水果产品进行了评估，以验证多重 PCR 测定的适用性。该测定有望成为检测食品中水果过敏原的特异性且有效的方法。