Lactobacillus brevis KU15153 was isolated from kimchi and probiotic characterization was performed including analysis of its antimicrobial and antioxidant effects. Lactobacillus rhamnosus GG (LGG) was used as a probiotic control. L. brevis KU15153 survived under artificial gastric conditions and was non-hemolytic, showed antibiotic susceptibility, and did not produce carcinogenic β-glucuronidase. L. brevis KU15153 adhered strongly to HT-29 cells in the direct adherent assay and showed high cell surface hydrophobicity. Particularly, L. brevis KU15153 showed antimicrobial activity against the food-borne pathogens Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 15313, Salmonella Typhimurium P99, and Staphylococcus aureus KCCM 11335. Antioxidant activity was assessed using the DPPH radical scavenging assay and β-carotene and linoleic acid inhibition assay. L. brevis KU15153 showed higher antioxidant activity than LGG. These results suggest that L. brevis KU15153 has potential for use as a probiotic organism.

中文翻译：

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短乳杆菌KU15153的益生菌特性显示出从泡菜中分离出的抗菌和抗氧化作用。

从泡菜中分离了短乳杆菌KU15153，并进行了益生菌表征，包括对其抗菌和抗氧化作用的分析。鼠李糖乳杆菌GG（LGG）用作益生菌对照。短乳杆菌KU15153在人工胃条件下存活并且不溶血，对抗生素敏感，并且不产生致癌的β-葡萄糖醛酸苷酶。在直接贴壁测定中，短乳杆菌KU15153与HT-29细胞牢固粘附，并显示出高的细胞表面疏水性。特别地，短乳杆菌KU15153对食源性病原菌大肠杆菌ATCC 25922，单核细胞增生李斯特菌ATCC 15313，鼠伤寒沙门氏菌P99和金黄色葡萄球菌KCCM 11335具有抗菌活性。使用DPPH自由基清除试验以及β-胡萝卜素和亚油酸抑制试验评估抗氧化活性。短乳杆菌KU15153显示出比LGG高的抗氧化活性。这些结果表明，短乳杆菌KU15153具有用作益生菌生物体的潜力。