Certain mechanical stimuli-particularly low-magnitude, high-frequency vibration-could induce bone marrow stem cell osteogenic differentiation and promote bone formation via Wnt signaling pathway, although the molecular mechanism is still unclear. In this study, we found that miR-335-5p is significantly upregulated after low-magnitude, high-frequency vibration, which suppresses the expression of the Wnt signaling inhibitor Dickkopf-related protein 1. Inhibition of miR-335-5p greatly reduced the osteogenic differentiation. Furthermore, the increase of miR-335-5p level was also confirmed in vivo after LMHF vibration in rabbit. Our study elucidates the prominent role of miRNAs that links the LMHF vibration and osteogenic differentiation.

中文翻译：

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低强度，高频振动通过增强miRNA-335-5p表达促进成骨分化。

某些机械刺激，特别是低振幅，高频振动，可能通过Wnt信号通路诱导骨髓干细胞成骨分化并促进骨形成，尽管其分子机制仍不清楚。在这项研究中，我们发现miR-335-5p在低强度，高频振动后显着上调，从而抑制了Wnt信号抑制剂Dickkopf相关蛋白1的表达。抑制miR-335-5p可以大大降低成骨分化。此外，在兔体内LMHF振动后，体内也证实了miR-335-5p水平的升高。我们的研究阐明了将LMHF振动和成骨分化联系起来的miRNA的显着作用。