In the present study, we investigated the effects of conditioned media (CM) collected from the cancer cell lines (K562, MCF-7, and HeLa) on peripheral blood mononuclear cells (PBMCs) isolated from the healthy human blood. The soluble factors in the CM are probably responsible for the differential mRNA expressions of Foxp3, Helios, Neuropilin- 1 (NRP-1), and glycoprotein A repetitions predominant (GARP), along with IFN-γ and TGF-β in PBMCs cultured with cancer cells CM. The PBMCs cultured with CM of K562 showed increased expression of Foxp3, Helios, NRP-1, GARP, IFN-γ, and TGF-β compared to PBMCs cultured with CM of MCF-7 and HeLa cells. In addition, the intracellular staining on PBMCs cultured with CM from cell lines were also evaluated for CD4, CD25, Foxp3, Helios, and NRP-1 by multicolor flow cytometry. The expression of CD4+CD25+Foxp3+, CD4+Helios+Foxp3+ and CD+NRP-1+Foxp3+ showed retarded cell population compared to control PBMCs. Our data suggest that soluble factors in CM of cancer cells may trigger the immune response in PBMCs resulting in a systematic response. Further research could lead to the identification of specific soluble factors that are involved in trafficking of cells into the immune cascades, which could be a safe and promising strategy for targeting human cancers.

中文翻译：

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在K562细胞培养的条件培养基（CM）中培养的外周血单个核细胞（PBMC）中TGF-β和IFN-γ的表达增加。

在本研究中，我们调查了从癌细胞系（K562，MCF-7和HeLa）收集的条件培养基（CM）对从健康人血中分离的外周血单个核细胞（PBMC）的影响。CM中的可溶性因子可能是Foxp3，Helios，Neuropilin-1（NRP-1）和主要糖蛋白A重复（GARP）以及IFN-γ和TGF-β的PBMC中mRNA差异表达的原因。癌细胞CM。与用MCF-7和HeLa细胞的CM培养的PBMC相比，用K562的CM培养的PBMC显示Foxp3，Helios，NRP-1，GARP，IFN-γ和TGF-β的表达增加。另外，还通过多色流式细胞术评估了用细胞系中的CM培养的PBMC的细胞内染色的CD4，CD25，Foxp3，Helios和NRP-1。CD4 + CD25 + Foxp3 +的表达，与对照PBMC相比，CD4 + Helios + Foxp3 +和CD + NRP-1 + Foxp3 +显示出受阻的细胞数量。我们的数据表明，癌细胞中CM中的可溶性因子可能会触发PBMC中的免疫反应，从而导致系统性反应。进一步的研究可能导致鉴定出与细胞向免疫级联中转运有关的特定可溶性因子，这可能是针对人类癌症的安全且有前途的策略。