Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast's function in gingival overgrowth. To determine whether amlodipine alters the inflammatory responses, we investigated its effects on gingival fibroblast gene expression as compared with untreated cells. Fragments of gingival tissue of healthy volunteers (11 years old boy, 68 years old woman, and 20 years old men) were collected during operation. Gene expression of 29 genes was investigated in gingival fibroblast cell culture treated with amlodipine, compared with untreated cells. Among the studied genes, only 15 (CCL1, CCL2D, CCL5, CCL8, CXCL5, CXCL10, CCR1, CCR10, IL1A, IL1B, IL5, IL7, IL8, SPP1, and TNFSF10) were significantly deregulated. In particular, the most evident overexpressed genes in treated cells were CCR10 and IL1A. These results seem to indicate a possible role of amlodipine in the inflammatory response of treated human gingival fibroblasts.

中文翻译：

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药物引起的牙龈增生：使用氨氯地平和人牙龈成纤维细胞的体外研究。

牙龈过度生长是氨氯地平的严重副作用。已经提出了几种相互矛盾的理论来解释成纤维细胞在牙龈过度生长中的功能。为了确定氨氯地平是否改变炎症反应，我们调查了其与未处理细胞相比对牙龈成纤维细胞基因表达的影响。手术期间收集健康志愿者（11岁男孩，68岁妇女和20岁男性）的牙龈组织碎片。与未处理的细胞相比，在用氨氯地平处理的牙龈成纤维细胞培养物中研究了29个基因的基因表达。在研究的基因中，只有15个（CCL1，CCL2D，CCL5，CCL8，CXCL5，CXCL10，CCR1，CCR10，IL1A，IL1B，IL5，IL7，IL8，SPP1和TNFSF10）被显着解除调节。特别是，在处理过的细胞中，最明显的过表达基因是CCR10和IL1A。这些结果似乎表明氨氯地平可能在治疗的人牙龈成纤维细胞的炎症反应中发挥作用。