Several distinct classes of drugs, such as anticonvulsants, immunosuppressants, and calcium channel blockers, caused gingival overgrowth. One of the main drugs associated with the gingival overgrowth is the anti-epileptic such as phenytoin, which affects gingival tissues by altering extracellular matrix metabolism. In our study, we evaluate the effect of phenytoin, a drug whose active substance is phenytoin, on gingival fibroblasts of healthy volunteers. Gene expression of 29 genes was investigated in gingival fibroblasts' cell culture treated with phenytoin compared with untreated cells. Among the studied genes, only 13 genes (CXCL5, CXCL10, CCR1, CCR3, CCR5, CCR6, IL-1A, IL-1B, IL-5, IL-7, IL-6R, BMP-2, and TNFSF-10) were statistically significant. All but one gene resulted downregulated after 24 h of treatment with phenytoin. BPM2 was the only, although weakly, up-expressed gene. Probably, we have not highlighted overexpression of the other inflammatory molecules because the study was performed on healthy people. Many studies show that phenytoin induces the overexpression of these cytokines but, probably, in our study, the drug does not have the same effect because we used gingival fibroblasts of healthy people.

中文翻译：

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苯妥英钠和牙龈粘膜：分子研究。

几种不同类别的药物（例如抗惊厥药，免疫抑制剂和钙通道阻滞剂）导致牙龈过度生长。与牙龈过度生长有关的主要药物之一是抗癫痫药，如苯妥英钠，它会通过改变细胞外基质代谢而影响牙龈组织。在我们的研究中，我们评估了苯妥英钠（一种活性物质为苯妥英钠）对健康志愿者的牙龈成纤维细胞的作用。与未处理的细胞相比，在用苯妥英钠处理的牙龈成纤维细胞的细胞培养物中研究了29个基因的基因表达。在研究的基因中，只有13个基因（CXCL5，CXCL10，CCR1，CCR3，CCR5，CCR6，IL-1A，IL-1B，IL-5，IL-7，IL-6R，BMP-2和TNFSF-10）具有统计学意义。苯妥英钠治疗24小时后，除一个基因外，所有基因均下调。BPM2是唯一但表达较弱的基因。由于这项研究是在健康人身上进行的，因此我们可能没有强调其他炎症分子的过度表达。许多研究表明苯妥英钠能诱导这些细胞因子的过度表达，但在我们的研究中，该药物可能没有相同的作用，因为我们使用了健康人的牙龈成纤维细胞。