The aim of present work is to assess the effects of bradykinin (Br) or noradrenaline (Nor) preconditioning to the levels of antioxidant enzymes: superoxide dismutase (SOD), copper, zinc superoxide dismutase (CuZn-SOD), manganese superoxide dismutase (Mn-SOD) and catalase in ischemia/reperfusion (I/R) model in the rabbit spinal cord white matter as well as effect on glial fibrillary acidic protein (GFAP) and ubiquitin immunoreaction in glial cells. Rabbits were preconditioned by intraperitoneal single dose of Br or Nor 48 h prior to 20 min of ischemia followed by 24 or 48 h of reperfusion. White matter of L3-L6 spinal cord segments was used for comparison of antioxidant enzyme levels in sham control, ischemic groups and four preconditioned groups. The total SOD level in the Br or Nor preconditioned groups after 48 h of reperfusion was increased vs Br or Nor preconditioned groups after 24 h of reperfusion. The comparison among the ischemic group vs Br preconditioned (P<0.05), and Nor preconditioned (P<0.001) groups after 48 h of reperfusion, showed statistically significant decrease of Mn-SOD activity. Tissue catalase level activity was significantly decreased in the Br preconditioned group after 48 h of reperfusion (P<0.05) and Nor preconditioned groups after 24 h of reperfusion (P<0.001) and also after 48 h of reperfusion (P<0.001), in comparison to ischemic group after 48 h of reperfusion. Significantly decreased tissue catalase activity (P<0.05) in both Nor preconditioned groups after 24 or 48 h of reperfusion was measured vs Br preconditioned group after 48 h of reperfusion. According to our results, in the white matter, activation of stress proteins in glial cells, as well as antioxidant enzymes levels, were influenced by pharmacological preconditioning followed by 20 min of ischemia and 24 or 48 h of reperfusion. These changes contribute to ischemic tolerance acquisition and tissue protection from oxidative stress during reperfusion period.

中文翻译：

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缓激肽和去甲肾上腺素预处理会影响兔缺血/再灌注后脊髓白质中抗氧化酶SOD，CuZn-SOD，Mn-SOD和过氧化氢酶的水平。

当前工作的目的是评估缓激肽（Br）或去甲肾上腺素（Nor）预处理对抗氧化酶水平的影响：超氧化物歧化酶（SOD），铜，锌超氧化物歧化酶（CuZn-SOD），锰超氧化物歧化酶（Mn -SOD）和过氧化氢酶在兔脊髓白质的缺血/再灌注（I / R）模型中，以及对神经胶质纤维酸性蛋白（GFAP）和神经胶质细胞泛素免疫反应的影响。在缺血20分钟之前，通过腹膜内单剂量Br或Nor预处理兔48小时，然后再灌注24或48 h。L3-L6脊髓节段的白质用于比较假手术对照组，缺血组和四个预处理组的抗氧化酶水平。再灌注48 h后，Br或Nor预处理组的总SOD水平高于再灌注24 h后的Br或Nor预处理组。缺血组与再灌注48 h后的Br预适应组（P <0.05）和Nor预适应组（P <0.001）之间的比较表明，Mn-SOD活性显着降低。再灌注48 h（P <0.05），再灌注24 h（P <0.001）和再灌注48 h（P <0.001）的Br预处理组的组织过氧化氢酶水平活性显着降低（P <0.05）。再灌注48小时后与缺血组比较。组织过氧化氢酶活性显着降低（P <0。05）在再灌注后24或48 h的两个Nor预处理组中均测得与再灌注后48 h的Br预处理组相比。根据我们的结果，在白质中，神经胶质细胞中应激蛋白的活化以及抗氧化酶的水平受到药理学预处理，缺血20分钟和再灌注24或48小时的影响。这些变化有助于在缺血再灌注期间获得缺血耐受性并保护组织免受氧化应激。