Fragmented data are available on the human polyomaviruses (HPyVs) prevalence in the gastrointestinal tract. Rearrangements in the non-coding control region (NCCR) of JCPyV and BKPyV have been extensively studied and correlated to clinical outcome; instead, little information is available for KIPyV, WUPyV and MCPyV NCCRs. To get insights into the role of HPyVs in the gastrointestinal tract, we investigated JCPyV, BKPyV, KIPyV, WUPyV and MCPyV distribution among hematological patients in concomitance with gastrointestinal symptoms. In addition, NCCRs and VP1 sequences were examined to characterize the strains circulating among the enrolled patients. DNA was extracted from 62 stool samples and qPCR was carried out to detect and quantify JCPyV, BKPyV, KIPyV, WUPyV and MCPyV genomes. Positive samples were subsequently amplified and sequenced for NCCR and VP1 regions. A phylogenetic tree was constructed aligning the obtained VP1 sequences to a set of reference sequences. qPCR revealed low viral loads for all HPyVs searched. Mono and co-infections were detected. A significant correlation was found between gastrointestinal complications and KIPyV infection. Archetype-like NCCRs were found for JCPyV and BKPyV, and a high degree of NCCRs stability was observed for KIPyV, WUPyV and MCPyV. Analysis of the VP1 sequences revealed a 99% identity with the VP1 reference sequences. The study adds important information on HPyVs prevalence and persistence in the gastrointestinal tract. Gastrointestinal signs were correlated with the presence of KIPyV, although definitive conclusions cannot be drawn. HPyVs NCCRs showed a high degree of sequence stability, suggesting that sequence rearrangements are rare in this anatomical site.

中文翻译：

---

血液病患者粪便中脱落的多瘤病毒：检测分析和非编码控制区遗传变异的研究。

有关胃肠道中人类多瘤病毒（HPyVs）患病率的零碎数据可用。已经广泛研究了JCPyV和BKPyV的非编码控制区（NCCR）中的重排，并与临床结果相关。相反，有关KIPyV，WUPyV和MCPyV NCCR的信息很少。为了深入了解HPyV在胃肠道中的作用，我们调查了伴有胃肠道症状的血液病患者中JCPyV，BKPyV，KIPyV，WUPyV和MCPyV的分布。另外，检查了NCCR和VP1序列以表征在所招募患者之间循环的菌株。从62个粪便样本中提取DNA，并进行qPCR检测和定量JCPyV，BKPyV，KIPyV，WUPyV和MCPyV基因组。随后扩增阳性样品，并对NCCR和VP1区进行测序。构建系统进化树，将获得的VP1序列与一组参考序列进行比对。qPCR显示所有搜索的HPyV病毒载量低。检测到单克隆和共感染。发现胃肠道并发症与KIPyV感染之间存在显着相关性。对于JCPyV和BKPyV，发现了原型原型NCCR，对于KIPyV，WUPyV和MCPyV，NCCR的稳定性很高。VP1序列的分析显示与VP1参考序列有99％的同一性。该研究增加了关于HPyV在胃肠道中的流行和持续性的重要信息。胃肠道体征与KIPyV的存在有关，尽管不能得出明确的结论。