Cyclophilin A (CypA) is an important host factor in the replication of a variety of RNA viruses. Also the replication of several nidoviruses was reported to depend on CypA, although possibly not to the same extent. These prior studies are difficult to compare, since different nidoviruses, cell lines and experimental set-ups were used. Here, we investigated the CypA dependence of three distantly related nidoviruses that can all replicate in Huh7 cells: the arterivirus equine arteritis virus (EAV), the alphacoronavirus human coronavirus 229E (HCoV-229E), and the betacoronavirus Middle East respiratory syndrome coronavirus (MERS-CoV). The replication of these viruses was compared in the same parental Huh7 cells and in CypA-knockout Huh7 cells generated using CRISPR/Cas9-technology. CypA depletion reduced EAV yields by ~ 3-log, whereas MERS-CoV progeny titers were modestly reduced (3-fold) and HCoV-229E replication was unchanged. This study reveals that the replication of nidoviruses can differ strikingly in its dependence on cellular CypA.

中文翻译：

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在细胞培养中复制过程中，冠状病毒和小动脉病毒在亲环蛋白A依赖性方面表现出惊人的差异。

亲环蛋白A（CypA）是复制各种RNA病毒的重要宿主因子。也有报道称几种尼多病毒的复制依赖于CypA，尽管可能程度不同。这些先前的研究很难进行比较，因为使用了不同的Nidovirus，细胞系和实验装置。在这里，我们研究了可以在Huh7细胞中复制的三种远距离相关的尼古病毒对CypA的依赖性：动脉病毒马动脉炎病毒（EAV），人冠状病毒人类冠状病毒229E（HCoV-229E）和β冠状病毒中东呼吸综合征冠状病毒（MERS） -CoV）。在相同的亲本Huh7细胞和使用CRISPR / Cas9技术生成的CypA敲除Huh7细胞中比较了这些病毒的复制。CypA耗竭使EAV产量降低了约3-log，而MERS-CoV后代效价则适度降低（3倍），而HCoV-229E复制保持不变。这项研究表明，尼杜病毒的复制在对细胞CypA的依赖性方面可以显着不同。