Mitochondria play essential roles in cell physiological processes including energy production, metabolism, ion homeostasis, cell growth, aging and apoptosis. Proteomic strategies have been applied to the study of mitochondria since 1998; these studies have yielded decisive information about the diverse physiological functions of the organelle. As an ideal model biological system, the nematode Caenorhabditis elegans has been widely used in the study of several diseases, such as metabolic diseases and cancer. However, the mitochondrial proteome of C. elegans remains elusive. In this study, we purified mitochondria from C. elegans and performed a comprehensive proteomic analysis using the shotgun proteomic approach. A total of 1117 proteins have been identified with at least two unique peptides. Their physicochemical and functional characteristics, subcellular locations, related biological processes, and associations with human diseases, especially Parkinson's disease, are discussed. An orthology comparison was also performed between C. elegans and four other model organisms for a general depiction of the conservation of mitochondrial proteins during evolution. This study will provide new clues for understanding the role of mitochondria in the physiological and pathological processes of C. elegans.

中文翻译：

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秀丽隐杆线虫线粒体的蛋白质组学分析。

线粒体在细胞生理过程中起着至关重要的作用，包括能量产生，代谢，离子稳态，细胞生长，衰老和凋亡。自1998年以来，蛋白质组学策略已应用于线粒体的研究。这些研究已得出有关细胞器多种生理功能的决定性信息。作为一种理想的模型生物学系统，线虫秀丽隐杆线虫已广泛用于研究多种疾病，例如代谢性疾病和癌症。但是，线虫的线粒体蛋白质组仍然难以捉摸。在这项研究中，我们从秀丽隐杆线虫中纯化了线粒体，并使用the弹枪蛋白质组学方法进行了全面的蛋白质组学分析。总共鉴定出1117种蛋白质，其中至少包含两种独特的肽。它们的理化和功能特性 讨论了亚细胞的位置，相关的生物学过程以及与人类疾病（尤其是帕金森氏病）的关系。在秀丽隐杆线虫和其他四个模型生物之间也进行了正交比较，以大致描述进化过程中线粒体蛋白的保守性。这项研究将提供新的线索，以了解线粒体在秀丽隐杆线虫的生理和病理过程中的作用。