Integral membrane proteins (IMPs) are difficult to identify, mainly for two reasons: the hydrophobicity of IMPs and their low abundance. Sample preparation is a key component in the large-scale identification of IMPs. In this review, we survey strategies for shotgun identification of IMPs by MS/MS. We will discuss enrichment, solubilization, separation, and digestion of IMPs, and data analysis for membrane proteomics.

中文翻译：

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串联质谱法用于shot弹枪鉴定完整膜蛋白的策略。

整体膜蛋白（IMPs）难以鉴定，主要有两个原因：IMPs的疏水性及其低丰度。样品制备是大规模鉴定IMP的关键组成部分。在这篇综述中，我们调查了通过MS / MS识别IMP的散弹枪识别策略。我们将讨论IMP的富集，增溶，分离和消化，以及膜蛋白质组学的数据分析。