Diagnostic methods for detecting Trypanosoma cruzi infection are important to allow administration of chemotherapy and as an experimental tool when trying to understand the pathogenesis of Chagas disease. A nested polymerase chain reaction (PCR)-based approach was recently used to demonstrate preferential heart and skeletal muscle tropism in mice of a Mexican T. cruzi isolate. The authors of this study also demonstrated higher sensitivity for this PCR setup compared with a commercially available enzyme-linked immunosorbent assay kit.

中文翻译：

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实验性南美锥虫病的分子诊断。

检测克鲁斯锥虫感染的诊断方法对于允许进行化疗以及作为尝试了解南美锥虫病发病机理的实验工具非常重要。最近使用基于巢式聚合酶链反应（PCR）的方法来证明墨西哥T. cruzi分离株小鼠的心脏和骨骼肌嗜性。与市售酶联免疫吸附测定试剂盒相比，该研究的作者还证明了此PCR设置的更高灵敏度。