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During stationary phase, Beijerinckia derxii shows nitrogenase activity concomitant with the release and accumulation of nitrogenated substances.
Microbiological Research ( IF 6.1 ) Pub Date : 2004-01-14 , DOI: 10.1078/0944-5013-00209
Natália Reiko Sato Miyasaka 1 , Daniela Strauss Thuler , Eny Iochevet Segal Floh , Walter Handro , Mariana Braga Duarte Toledo , Sônia Maria Gagioti , Heloiza Ramos Barbosa
Affiliation  

Beijerinckia derxii, a free-living nitrogen-fixing bacterium, maintained an increasing nitrogenase specific activity during the stationary growth phase. To verify the destination of the nitrogen fixed during this phase, intra and extracellular nitrogenated contents were analyzed. Organic nitrogen and amino acids were detected in the supernatant of the cultures. An increase in intracellular content of both nitrogen and protein occurred. Cytoplasmic granules indicated the presence of arginine. The ability of a non-diazotrophic bacterium (E. coli) to use B. derxii proteins as a source of nitrogen was observed concomitantly with E. coli growth. There is a suggestion that B. derxii contributes to the environment by both releasing nitrogenated substances and accumulating substances capable of being consumed after its death.

中文翻译:

在静止期,德氏贝氏菌显示出硝化酶活性,并伴随着硝化物质的释放和积累。

自由生存的固氮细菌Beijerinckia derxii在固定生长期期间保持着增加的固氮酶比活性。为了验证在此阶段固定的氮的目的地,分析了细胞内和细胞外的含氮量。在培养物的上清液中检测到有机氮和氨基酸。氮和蛋白质的细胞内含量均增加。细胞质颗粒表明存在精氨酸。伴随着大肠杆菌的生长,观察到非重营养细菌(E. coli)使用皮肤芽孢杆菌蛋白质作为氮源的能力。有建议认为,der.i。B.通过释放氮化物和积累能够在其死亡后消耗的物质而对环境有贡献。
更新日期:2019-11-01
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