Frequency of proteinase 3 (PR3)-specific autoreactive T cells determined by cytokine flow cytometry in Wegener's granulomatosis.,Journal of Autoimmunity

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Frequency of proteinase 3 (PR3)-specific autoreactive T cells determined by cytokine flow cytometry in Wegener's granulomatosis.
Journal of Autoimmunity ( IF 12.8 ) Pub Date : 2004-01-08 , DOI: 10.1016/j.jaut.2003.10.008
Jolanta Winek ₁ , Antje Mueller , Elena Csernok , Wolfgang L Gross , Peter Lamprecht

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OBJECTIVE Previous studies have shown proliferation to Wegener's autoantigen, proteinase 3 (PR3). We tested immunogenicity of PR3-derived peptides and determined frequencies of PR3-specific T cells using cytokine flow cytometry in Wegener's granulomatois (WG). METHODS Peripheral blood T-cell responses were measured after stimulation with previously described PR3-derived peptides. PBMC were stimulated with PR3-derived peptides or control stimuli for up to 10 days. Cells were stained with antibodies against CD4 or CD8, CD69 and intracellular TNF-alpha and analyzed by flow cytometry. PR3-specific T cells were counted as the percentage of CD69(+)TNF-alpha(+)double positive T cells after stimulation. RESULTS Frequencies of PR3-specific peripheral blood T cells after short-term stimulation (

中文翻译：

通过细胞因子流式细胞术确定的韦格纳肉芽肿病中的蛋白酶3（PR3）特异性自身反应性T细胞的频率。

目的以前的研究表明，韦格纳自身抗原蛋白酶3（PR3）具有增殖作用。我们在韦格纳肉芽肿（WG）中使用细胞因子流式细胞仪测试了PR3衍生肽的免疫原性，并确定了PR3特异性T细胞的频率。方法用先前描述的PR3衍生肽刺激后测量外周血T细胞反应。PBMC用PR3衍生的肽或对照刺激刺激长达10天。用针对CD4或CD8，CD69和细胞内TNF-α的抗体对细胞染色，并通过流式细胞术进行分析。PR3特异性T细胞计数为刺激后CD69（+）TNF-α（+）双阳性T细胞的百分比。结果短期刺激后PR3特异性外周血T细胞的频率（

更新日期：2019-11-01

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