Celiac disease is characterized by intestinal mucosal injury and malabsorption precipitated by dietary exposure to gluten of some cereals with a prominent role being played by gliadins, specific antigenic determinants found in wheat gluten. Patients suffering from celiac disease have serum antibodies recognizing gliadin, as well as the endomysial autoantigen tissue transglutaminase. Phage display antibody libraries have revealed ectopic production of anti-transglutaminase antibodies by intestinal lymphocytes with a biased use of the VH5 antibody gene family. Here we report a study on the pairing of VH and VL families in the antibodies to transglutaminase. Our results led to the construction of small phage display antibody libraries based on the amplification of the two genes in the VH5 family from intestinal lymphocytes. This method can be used for the rapid characterization of the anti-transglutaminase response in a potentially large number of subjects including asymptomatic patients whose serum antibodies may be undetectable.

中文翻译：

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从患有乳糜泻的受试者中一步克隆抗组织转谷氨酰胺酶 scFv。

乳糜泻的特征是肠道黏膜损伤和吸收不良，这是由于膳食暴露于某些谷物的麸质而导致的，其中麦醇溶蛋白（小麦麸质中发现的特定抗原决定因素）发挥着重要作用。患有乳糜泻的患者具有识别麦醇溶蛋白以及肌内膜自身抗原组织转谷氨酰胺酶的血清抗体。噬菌体展示抗体文库揭示了肠道淋巴细胞异位产生抗转谷氨酰胺酶抗体，并偏向使用 VH5 抗体基因家族。在这里，我们报告了一项关于转谷氨酰胺酶抗体中 VH 和 VL 家族配对的研究。我们的结果导致基于从肠淋巴细胞中扩增 VH5 家族中的两个基因构建小型噬菌体展示抗体文库。