De novo protein design has created small, robust protein-cofactor complexes that serve as simplified working models, or maquettes, for the much more complicated natural oxidoreductases. We review the research avenues that spring from the better characterized water-soluble hydrophilic maquettes and guide the construction of amphiphilic maquettes patterned on membrane-bound oxidoreductases that couple electron transfer to transmembrane proton-motive force. We address the special working challenges and opportunities that arise with amphiphilic maquettes designed to assemble in membranes, along with the redox and pigment cofactors required to promote light activated electron transfer, redox-coupled electric field generation, proton exchange and transmembrane charge motion.

中文翻译：

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氧化还原蛋白模型的亲水性至两亲性设计。

从头进行的蛋白质设计已经创建了小的，坚固的蛋白质辅因子复合物，可用于简化复杂的天然氧化还原酶的工作模型或模型。我们回顾了从性能更好的水溶性亲水性模型中获得的研究途径，并指导在膜结合的氧化还原酶上进行构图的两亲性模型的构建，该膜结合的氧化还原酶将电子传递耦合到跨膜质子动力上。我们解决了设计成在膜中组装的两亲模型的特殊工作挑战和机遇，以及促进光活化电子转移，氧化还原耦合电场产生，质子交换和跨膜电荷运动所需的氧化还原和色素辅因子。