Enzymes with the (beta/alpha)(8)-barrel fold are involved in the catalysis of a wide variety of biochemical reactions. The active sites of these enzymes are located on the C-terminal face of the central beta-barrel. Conserved amino acid sequence, as well as secondary, tertiary and quaternary structure patterns are providing a rich body of data to support the premise of a common ancestry of many members of the (beta/alpha)(8)-barrel fold family of enzymes. Recent data indicate that there is at least one example of a bienzyme that functions as an ammonia channel, adding a new level of functional diversity to the (beta/alpha)(8)-barrel fold. These proteins have become ideal tools that can be used in conjunction with directed evolution techniques to engineer novel catalytic activities.

中文翻译：

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（beta / alpha）8桶折叠中的进化标记。

具有（beta / alpha）（8）-barfold折叠的酶参与多种生化反应的催化。这些酶的活性位点位于中央β-桶的C末端面上。保守的氨基酸序列，以及二级，三级和四级结构模式提供了丰富的数据，以支持（beta / alpha）（8）-桶折叠家族的许多成员的共同祖先的前提。最近的数据表明，至少有一个双酶作为氨通道的例子，为（beta / alpha）（8）-桶状折叠增加了新的功能多样性。这些蛋白质已成为理想的工具，可与定向进化技术结合使用以工程化新的催化活性。