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GFP technology for live cell imaging.
Current Opinion in Plant Biology ( IF 8.3 ) Pub Date : 2003-11-13 , DOI: 10.1016/j.pbi.2003.09.014
David Ehrhardt 1
Affiliation  

Fluorescent proteins are generating fresh insight into plant cell function by providing new opportunities to visualize structure and dynamic events in live cells. Novel and transient structures, such as discreet locations in the nucleus where activated photoreceptor proteins accumulate, have recently been identified with fluorescent protein tags. Fluorescent proteins have also enabled the discovery of new dynamic molecular behaviors, such as the repositioning of cortical microtubules by polymer treadmilling. The early potential of fluorescent proteins to reveal protein interactions in living cells is being realized, as demonstrated in recent studies of transcription factors and signal transduction proteins. A promising new approach to the creation of fluorescent-protein-based biosensors has produced an exciting family of tools for visualizing small sugars, and perhaps will produce a wide variety of other small molecules in the future.

中文翻译:

用于活细胞成像的GFP技术。

荧光蛋白通过提供可视化活细胞结构和动态事件的新机会,正在对植物细胞功能产生新的见解。最近已经用荧光蛋白标签鉴定了新颖的和短暂的结构,例如核中离散的位置,活化的感光蛋白在其中积累。荧光蛋白还使人们能够发现新的动态分子行为,例如通过聚合物跑步机重新定位皮质微管。正如最近对转录因子和信号转导蛋白的研究所证明的那样,荧光蛋白具有揭示活细胞中蛋白相互作用的早期潜力。
更新日期:2019-11-01
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