当前位置: X-MOL 学术J. Basic Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Analysis of genome sequence and trehalose lipid production peculiarities of the thermotolerant Gordonia strain
Journal of Basic Microbiology ( IF 3.5 ) Pub Date : 2019-11-07 , DOI: 10.1002/jobm.201900439
Yanina Delegan 1, 2 , Armen Sargsyan 3 , Nelli Hovhannisyan 3, 4 , Bella Babayan 3 , Kirill Petrikov 1, 2 , Mikhail Vainstein 1, 2
Affiliation  

Gordoniae are one of the most promising hydrocarbon‐oxidizing actinobacteria. Here we present the genome sequence analysis of thermotolerant strain Gordonia sp. 1D isolated from oil‐refinery soil. It is capable of alkane consumption and biosurfactant production at temperatures of up to 50°C. Gordonia sp. 1D demonstrates maximum biosurfactant production when grown on hexadecane, and at 40°C it was slightly higher than at 27°C: 35 and 39 mN/m, respectively. For the first time, it was experimentally confirmed that the carbohydrate component of extracellular biosurfactants produced by strain 1D is trehalose. In addition, genes for the production of trehalose lipid biosurfactants were identified. The genetic determinants for two different pathways for trehalose synthesis were found. The strain carries genes otsA and otsB involved in de novo trehalose biosynthesis. Moreover, the genes treY and treZ responsible for trehalose biosynthesis from maltooligosaccharides and starch or glycogen were identified.

中文翻译:

耐热Gordonia菌株基因组序列及海藻糖脂质生产特性分析

Gordoniae 是最有前途的烃氧化放线菌之一。在这里,我们介绍了耐热菌株 Gordonia sp. 的基因组序列分析。从炼油厂土壤中分离出来的一维。它能够在高达 50°C 的温度下消耗烷烃和生产生物表面活性剂。戈多尼亚 sp. 图 1D 展示了在十六烷上生长时生物表面活性剂的最大产量,并且在 40°C 下比在 27°C 下略高:分别为 35 和 39 mN/m。首次通过实验证实1D菌株产生的细胞外生物表面活性剂的碳水化合物成分为海藻糖。此外,还鉴定了用于生产海藻糖脂质生物表面活性剂的基因。发现了海藻糖合成的两种不同途径的遗传决定因素。该菌株携带参与海藻糖从头生物合成的基因 otsA 和 otsB。此外,还鉴定了负责从麦芽低聚糖和淀粉或糖原生物合成海藻糖的基因 treY 和 treZ。
更新日期:2019-11-07
down
wechat
bug