Onion (Allium cepa) and leek (Allium ampeloprasum var. porrum) are common herbs and vegetables found in our daily life. It belongs to the genus Allium, which is usually known for their high antioxidant and anticancer properties. Medical researchers highly recommend the exploitation of herbs and plants as alternative ways in the treatment of cancer. This research was designed to study the anticancer effects of onion and leek extracts on MCF-7 human breast cancer cell. Crude extracts of ethanol, methanol, and water of onion and leek were obtained by maceration. MCF-7 cells were cultured in complete media at 37 °C and subjected to different treatments that involved varying concentrations (10, 50, and 100 μg/mL) of onion and leek extracts for 24, 48, and 72 h of incubation. The percentage of cell viability and the concentration of extracts on MCF-7 cells were determined using MTT assay. The water leek extract proved to be the most effective extract at 50 μg/mL, whereby it showed a significant inhibition ability due to the presence of entadamide A-β-D-glucopyranoside as identified by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF/MS). Further studies about the mechanism of both extracts in causing cell death and the determination of the presence of other bioactive compounds in the extracts are needed.

中文翻译：

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洋葱 (Allium cepa) 和韭菜 (Allium ampeloprasum) 的比较研究：UPLC-QTOF/MS 鉴定有机硫化合物以及对 MCF-7 细胞的抗癌作用

洋葱 (Allium cepa) 和韭菜 (Allium ampeloprasum var. porrum) 是我们日常生活中常见的草药和蔬菜。它属于葱属，通常以其高抗氧化和抗癌特性而闻名。医学研究人员强烈建议开发草药和植物作为治疗癌症的替代方法。本研究旨在研究洋葱和韭菜提取物对 MCF-7 人乳腺癌细胞的抗癌作用。通过浸渍获得洋葱和韭葱的乙醇、甲醇和水的粗提取物。MCF-7 细胞在 37 °C 的完全培养基中培养，并进行不同处理，包括不同浓度（10、50 和 100 μg/mL）的洋葱和韭菜提取物，孵育 24、48 和 72 小时。使用 MTT 法测定细胞活力的百分比和 MCF-7 细胞上提取物的浓度。水韭菜提取物在 50 μg/mL 时被证明是最有效的提取物，由于超高效液相色谱-四极杆/时间-鉴定的恩他他胺 A-β-D-吡喃葡萄糖苷的存在，它显示出显着的抑制能力。飞行质谱 (UPLC-QTOF/MS)。需要进一步研究这两种提取物导致细胞死亡的机制，以及确定提取物中是否存在其他生物活性化合物。通过超高效液相色谱-四极杆/飞行时间质谱 (UPLC-QTOF/MS) 鉴定，由于恩他他胺 A-β-D-吡喃葡萄糖苷的存在，它显示出显着的抑制能力。需要进一步研究这两种提取物导致细胞死亡的机制，以及确定提取物中是否存在其他生物活性化合物。通过超高效液相色谱-四极杆/飞行时间质谱 (UPLC-QTOF/MS) 鉴定，由于恩他他胺 A-β-D-吡喃葡萄糖苷的存在，它显示出显着的抑制能力。需要进一步研究这两种提取物导致细胞死亡的机制，以及确定提取物中是否存在其他生物活性化合物。