Fusarium pseudograminearum is an important pathogen of Fusarium crown rot and Fusarium head blight, which is able to infect wheat and barley worldwide, causing great economic losses. Transcription factors (TFs) of the basic leucine zipper (bZIP) protein family control important processes in all eukaryotes. In this study, we identified a gene, designated FpAda1, encoding a bZIP TF in F. pseudograminearum. The homolog of FpAda1 is also known to affect hyphal growth in Neurospora crassa. Deletion of FpAda1 in F. pseudograminearum resulted in defects in hyphal growth, mycelial branching and conidia formation. Pathogenicity assays showed that virulence of the Δfpada1 mutant was dramatically decreased on wheat coleoptiles and barley leaves. However, wheat coleoptile inoculation assay showed that Δfpada1 could penetrate and proliferate in wheat cells. Moreover, the FpAda1 was required for abnormal nuclear morphology in conidia and transcription of FpCdc2 and FpCdc42. Taken together, these results indicate that FpAda1 is an important transcription factor involved in growth and development in F. pseudograminearum.

中文翻译：

---

bZIP转录因子FpAda1对假单胞镰刀菌的真菌生长和分生至关重要。

伪镰刀菌是镰刀菌冠腐病和镰刀菌病的重要病原体，能够感染全世界的小麦和大麦，造成巨大的经济损失。碱性亮氨酸拉链（bZIP）蛋白家族的转录因子（TFs）控制着所有真核生物的重要过程。在这项研究中，我们鉴定了一个基因，命名为FpAda1，在假单胞菌中编码bZIP TF。还已知FpAda1的同源物会影响神经孢菌的菌丝生长。假单胞菌中FpAda1的缺失导致菌丝生长，菌丝体分支和分生孢子形成缺陷。致病性分析表明，在小麦胚芽鞘和大麦叶片上，Δfpada1突变体的毒力显着降低。然而，小麦胚芽鞘接种试验表明Δfpada1可以穿透并在小麦细胞中增殖。此外，FpAda1是分生孢子中异常核形态和FpCdc2和FpCdc42转录所必需的。综上所述，这些结果表明FpAda1是参与假单胞菌生长和发育的重要转录因子。