This study aimed to explore the influence of Tryptophanyl-tRNA synthetase (WARS) expression on the proliferation and migration of uveal melanoma (UM) cells, and the potential mechanisms. Bioinformatics analysis based on Gene Expression Omnibus (GEO) database showed that WARS expression in metastatic cancer was significantly higher than that in no-metastatic group. Kaplan-Meier analysis based on The Cancer Genome Atlas (TCGA) database showed that high WARS expression was associated with lower survival. Biological function experiments showed that overexpression of WARS in OCM-1A cells can promote cell proliferation, migration, and invasion, whereas knockdown of WARS in C918 cells showed the opposite effect. Finally, we observed that the up-regulation of WARS induced the activation of phosphatidylinositol 3-kinase/AKT (PI3K/AKT) signaling, whilst depletion of WARS resulted in opponent outcomes. Taken together, our results illustrated that WARS was overexpressed in UM cells and contributed to the viability and motility of UM cells via modulating PI3K/AKT signaling pathway.

中文翻译：

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葡萄膜黑色素瘤中的色氨酸tRNA合成酶（WARS）表达-葡萄膜黑色素瘤进展过程中的可能贡献者。

这项研究旨在探讨色氨酸-tRNA合成酶（WARS）表达对葡萄膜黑色素瘤（UM）细胞增殖和迁移的影响及其潜在机制。基于基因表达综合数据库（GEO）的生物信息学分析表明，转移癌中的WARS表达显着高于无转移组。基于癌症基因组图谱（TCGA）数据库的Kaplan-Meier分析表明，高WARS表达与较低的生存率相关。生物学功能实验表明，WARS在OCM-1A细胞中的过表达可以促进细胞增殖，迁移和侵袭，而敲低C918细胞中的WARS则表现出相反的作用。最后，我们观察到WARS的上调诱导了磷脂酰肌醇3-激酶/ AKT（PI3K / AKT）信号的激活，而消耗WARS则导致对手结局。两者合计，我们的结果表明，WARS在UM细胞中过表达，并通过调节PI3K / AKT信号通路对UM细胞的活力和运动作出贡献。