Background: T regulatory cells (Tregs), through variable mechanisms, play a crucial role in Hepatitis C virus (HCV) chronicity and infection tolerance. A great speculation is posed regarding the level, role of Tregs in end-stage renal disease (ESRD), and the presence of associated factors that could influence the Tregs population. Accordingly, we aimed at studying the effect of HCV infection on peripheral CD4+CD25+Tregs population among patients on hemodialysis (HD) as well as the effect of other comorbidities on these cells.Patients and methods: A group of 77 patients on HD (32 were HD HCV+ and 45 were HD HCV-) and 80 healthy controls (HCs) were included in the study. Flow cytometric analysis was performed for identification and quantification of peripheral CD4+ CD25+Tregs.Results: The frequency of CD4+ CD25+Tregs increased significantly in HD patients compared to the HCs (p = <.0001 each). HCV posed no effect on peripheral CD4+ CD25+ Tregs in ESRD patients, when comparing HD HCV- and HD HCV+ groups. In the hypertensive HD HCV-, Tregs percentage was higher than that in the non-hypertensive. However, the difference was not statistically significant. No significant difference was detected between HD HCV- and HD HCV+ patients on the count and percentages of Tregs according to the duration of dialysis.Conclusion: Demonstrating that chronic HCV infection has no effect on CD4+ CD25+ Tregs cells levels in ESRD patients are of great importance to the success of future allografts in such patients.

中文翻译：

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慢性丙型肝炎感染对终末期肾脏疾病患者的外周CD4 + CD25 +调节细胞没有影响。

背景：T调节细胞（Tregs）通过多种机制在丙型肝炎病毒（HCV）的慢性和感染耐受性中发挥关键作用。人们对Tregs在终末期肾脏疾病（ESRD）中的水平，作用以及可能影响Tregs人群的相关因素的存在提出了很大的推测。因此，我们旨在研究HCV感染对血液透析（HD）患者外周CD4 + CD25 + Tregs的影响以及其他合并症对这些细胞的影响。患者和方法：一组77位HD患者（研究中包括32例HD HCV +和45例HD HCV-。进行流式细胞仪分析以鉴定和定量外周CD4 + CD25 + Tregs。与HC相比，HD患者中CD4 + CD25 + Treg的频率显着增加（每个p = <.0001）。比较HD HCV-和HD HCV +组时，HCV对ESRD患者的外周CD4 + CD25 + Treg没有影响。在高血压HD HCV-中，Tregs百分比高于非高血压患者。但是，差异没有统计学意义。根据透析时间，HD HCV-和HD HCV +患者之间的Treg数量和百分比没有显着差异。结论：证明慢性HCV感染对ESRD患者的CD4 + CD25 + Tregs细胞水平没有影响。将来在此类患者中成功移植。比较HD HCV-和HD HCV +组时。在高血压HD HCV-中，Tregs百分比高于非高血压患者。但是，差异没有统计学意义。根据透析时间，HD HCV-和HD HCV +患者之间的Treg数量和百分比没有显着差异。结论：证明慢性HCV感染对ESRD患者的CD4 + CD25 + Tregs细胞水平没有影响。将来在此类患者中成功移植。比较HD HCV-和HD HCV +组时。在高血压HD HCV-中，Tregs百分比高于非高血压患者。但是，差异没有统计学意义。根据透析时间，HD HCV-和HD HCV +患者之间的Treg数量和百分比没有显着差异。结论：证明慢性HCV感染对ESRD患者的CD4 + CD25 + Tregs细胞水平没有影响。将来在此类患者中成功移植。