Abstract

Peptic ulcer disease (PUD) is an important cause of morbidity and mortality throughout the world affecting lives of millions of people. Hyssopus officinalis L. have been used as carminative and antispasmodic stomachic in Iran and Indian traditional systems of medicine. Thus, present study was aimed to evaluate gastroprotective activity of ethanolic extract of Hyssopus officinalis L. leaves (EEHO) in indomethacin-induced gastric ulcer in experimental rats. Female Sprague Dawley rats of groups I, II, III, IV, V, and VI received orally 1 mL/kg/day 1% CMC (carboxy methylcellulose), 1 mL/kg/day 1% CMC, 250 mg EEHO/kg/day, 500 mg EEHO/kg/day, 50 mg ranitidine/kg/day and 500 mg EEHO/kg/day respectively for 10 days. Then, all the groups except groups I and VI were orally administered with 20 mg indomethacin/kg b.wt on 11th day. Ulcer index and mucus barrier were determined. Antioxidant parameters thiobarbituric acid reactive substances (TBARS), glutathione-reduced (GSH), catalase and superoxide dismutase (SOD) were evaluated. Stomach was examined for histopathology also. EEHO in groups III and IV significantly (p < 0.01) increased the mucus barrier, SOD, GSH, and catalase while significantly (p < 0.01) decreased the ulcer index and TBARS as compared to ulcer control group II. Histopathological findings showed that indomethacin administration in group II caused PUD (gastric ulcer) and the gastric ulcer was protected by pretreatment with EEHO in groups III and IV. Thus, EEHO possesses gastroprotective activity where the gastroprotection is by strengthening of the gastric mucosa and reduction of oxidative stress. The gastroprotective activity of EEHO was comparable to that of standard drug ranitidine.

摘要

消化性溃疡病 (PUD) 是影响全世界数百万人生活的发病率和死亡率的重要原因。Hyssopus officinalis L. 在伊朗和印度传统医学体系中被用作驱风剂和解痉健胃剂。因此，本研究旨在评估海索草乙醇提取物的胃保护活性。消炎痛诱导的实验大鼠胃溃疡中的 L. 叶 (EEHO)。I、II、III、IV、V 和 VI 组的雌性 Sprague Dawley 大鼠口服 1 mL/kg/天 1% CMC（羧甲基纤维素）、1 mL/kg/天 1% CMC、250 mg EEHO/kg/天、500 mg EEHO/kg/天、50 mg 雷尼替丁/kg/天和 500 mg EEHO/kg/天分别持续 10 天。然后，除I组和VI组之外的所有组在第11天口服给予20mg吲哚美辛/kg b.wt。测定溃疡指数和粘液屏障。评估了抗氧化参数硫代巴比妥酸反应物质 (TBARS)、谷胱甘肽还原 (GSH)、过氧化氢酶和超氧化物歧化酶 (SOD)。还检查了胃的组织病理学。III 组和 IV 组的 EEHO 显着 ( p < 0.01) 增加粘液屏障、SOD、GSH 和过氧化氢酶，同时显着 ( p  < 0.01) 降低溃疡指数和 TBARS 与溃疡对照组 II 相比。组织病理学结果显示，II 组中的消炎痛给药引起 PUD（胃溃疡），并且胃溃疡在 III 和 IV 组中通过 EEHO 预处理得到保护。因此，EEHO具有胃保护活性，其中胃保护是通过增强胃粘膜和减少氧化应激来实现的。EEHO 的胃保护活性与标准药物雷尼替丁相当。