Campylobacteriosis is one of the most frequently reported zoonoses worldwide. The well-documented increase in the ciprofloxacin resistance has increased the importance of rapid detection of the resistance. The incidence of ciprofloxacin resistance was investigated using real-time PCR. Identification of one hundred and fifty-eight strains was performed by PCR. Minimum inhibitory concentration (MIC) of ciprofloxacin was determined by Epsilometer test. Following the confirmation of the efficiencies of singleplex real-time PCR methods using two different probes, a cytosine to thymine point mutation at codon 86 was detected by allelic discrimination. Of the 158 strains, 114 (72.2%) were determined to be resistant to ciprofloxacin. The MIC50 and the MIC90 of ciprofloxacin were found to be 8 and ≥32 mg/L, respectively. By real-time PCR, the presence of the mutation was confirmed in all, but one, resistant strains and the absence of the mutation was demonstrated in all, but one, susceptible strains. The rate of resistance is high among C. jejuni strains and ciprofloxacin should not be used in the treatment of such infections in Turkey. A cytosine to thymine mutation is the most frequently detected mechanism for the resistance. Real-time PCR can be used for the quick screening of the resistance.

中文翻译：

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人类空肠弯曲杆菌菌株对环丙沙星耐药性的分子和表型研究：土耳其耐药性高发。

弯曲杆菌病是世界上最常报道的人畜共患病之一。环丙沙星耐药性的有据可查的增加增加了快速检测耐药性的重要性。使用实时PCR研究了环丙沙星耐药性的发生率。通过PCR鉴定了158个菌株。环丙沙星的最小抑菌浓度（MIC）通过Epsilometer测试确定。在确认使用两种不同探针的单重实时PCR方法的效率后，通过等位基因鉴别检测到了第86位密码子的胞嘧啶至胸腺嘧啶点突变。在158株菌株中，有114株（72.2％）被确定对环丙沙星耐药。环丙沙星的MIC50和MIC90分别为8和≥32mg / L。通过实时PCR，在所有但仅一种抗性菌株中证实了突变的存在，而在除一种易感菌株中所有突变菌株中均证实了突变的缺失。空肠弯曲杆菌菌株中的耐药率很高，在土耳其不应使用环丙沙星治疗此类感染。胞嘧啶向胸腺嘧啶突变是最常见的耐药机制。实时PCR可用于快速筛选抗性。