Bivalve mollusks as filter-feeders concentrate in their digestive tissue microorganisms likely present in the harvesting water, thus becoming risky food especially if consumed raw or poorly cooked. To eliminate bacteria and viruses eventually accumulated, they must undergo a depuration process which efficacy on viruses is on debate. To better clarify the worth of the depuration process on virus elimination from mussels, in this study we investigated rotavirus kinetics of accumulation and depuration in Mytilus galloprovincialis experimentally contaminated. Depuration process was monitored for 9 days and virus residual presence and infectivity were evaluated by real time quantitative polymerase chain reaction, cell culture and electron microscopy at days 1, 2, 3, 5, 7, 9 of depuration. Variables like presence of ozone and of microalgae feeding were also analyzed as possible depuration enhancers. Results showed a two-phase virus removal kinetic with a high decrease in the first 24 h of depuration and 5 days necessary to completely remove rotavirus.

中文翻译：

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轮状病毒在受实验污染的贻贝中的积累和净化动力学。

双壳软体动物作为滤食动物集中在其可能存在于集水的消化组织微生物中，因此成为有风险的食物，特别是如果食用生食或烹饪不佳的食物。为了消除最终积累的细菌和病毒，它们必须经过净化过程，该过程对病毒的功效尚存争议。为了更好地阐明净化过程对消除贻贝中的病毒的价值，在这项研究中，我们调查了轮状病毒在盖氏菌中的积累和净化动力学。受实验污染。监测净化过程9天，并在净化的第1、2、3、5、7、9天通过实时定量聚合酶链反应，细胞培养和电子显微镜评估病毒残留的存在和感染性。还分析了诸如臭氧和微藻饲料的存在之类的变量作为可能的净化促进剂。结果显示两阶段病毒去除动力学，在净化的最初24小时内有很高的降低，而完全去除轮状病毒则需要5天。