Selective adhesion and directional migration of endothelial cells (ECs) on biomaterials is critical to realize the rapid endothelialization. In this study, a Cys‐Ala‐Gly (CAG) peptide density gradient is generated on homogeneous cell‐resisting poly(2‐hydroxyethyl methacrylate‐co‐glycidyl methacrylate) brushes by immersing the brushes in a complementary gradient solution of CAG and competitive mercapto‐terminated methoxyl poly(ethylene glycol). The adhesion and spreading of smooth muscle cells (SMCs) is impaired effectively on the gradient surface. About six folds of adherent ECs over SMCs are achieved at the position (10 mm) of highest CAG density on the gradient surface in a co‐culture condition. Due to the gradient cues, ECs migrate fastest with the best directionality of 86.7% at the middle of the gradient, leading to the maximum net displacement as well.

中文翻译：

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Cys-Ala-Gly肽密度梯度在防污聚合物刷上引导内皮细胞的选择性粘附和定向迁移。

内皮细胞（ECs）在生物材料上的选择性粘附和定向迁移对于实现快速内皮化至关重要。在这项研究中，通过将均质的抗细胞聚甲基丙烯酸2-羟乙酯-甲基丙烯酸缩水甘油酯共聚物刷浸入CAG和竞争性巯基的互补梯度溶液中，生成了Cys-Ala-Gly（CAG）肽密度梯度。封端的甲氧基聚乙二醇。平滑肌细胞（SMCs）的粘附和扩散会在梯度表面上受到有效削弱。在共培养条件下，在梯度表面上最高CAG密度的位置（10毫米）处，附着的EC达到SMC的六倍。由于存在梯度提示，EC迁移速度最快，在梯度中间的最佳方向性为86.7％，