Mesenchymal stem cells (MSCs) are a promising tool for cell therapy, and gene-modified MSCs further expand their applications. To take full advantage of MSCs as a therapeutic approach, developing effective gene transfer methods is critical. Calcium phosphate transfection is well-established and safe, but the protocols need to be optimized according to different cell types. Currently, there is no optimized protocol for MSCs. This study optimized the protocol of calcium phosphate transfection for MSCs and highlighted the importance of serum during the process of transfection. More interestingly, the behavior of gene overexpression in MSCs in the in vivo environment was verified.

中文翻译：

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间充质干细胞磷酸钙转染的优化和表征。


间充质干细胞（MSC）是一种有前景的细胞治疗工具，基因修饰的 MSC 进一步扩展了其应用。为了充分利用间充质干细胞作为治疗方法，开发有效的基因转移方法至关重要。磷酸钙转染是成熟且安全的，但需要根据不同的细胞类型优化方案。目前，还没有针对 MSC 的优化方案。本研究优化了MSCs的磷酸钙转染方案，并强调了血清在转染过程中的重要性。更有趣的是，验证了MSCs在体内环境中基因过度表达的行为。