Sixty-six strains classified as P. verrucosum based on morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains in the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate from plants. The ribosomal ITS1-5.8S-ITS2 sequences were similar, except for two single nucleotide exchanges in several strains of each group. A chemotaxonomical analysis of some of the strains identified differences between the groups in secondary metabolite production. Strains from group I possessed the chemotype of P. nordicum and strains from group II that of P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either P. verrucosum or P. nordicum. The homolgy between the ITS sequences however indicates phylogenetic relationship between the two species.

中文翻译：

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青霉属产生曲霉毒素A的菌株的分子表征。

根据RAPD，AFLP和ITS测序等分子方法，对根据形态学标准分类为疣状假单胞菌的66株菌株进行了鉴定。通过RAPD和AFLP分析可以确定两组。两个RAPD以及两个AFLP组完全是巧合的。两组中的菌株产生曲霉毒素A的能力有所不同，第一组主要包含高产菌株，第二组含有中度至非生产菌株。来自第一组的菌株源自食物，例如奶酪和肉制品，来自第二组的菌株源自植物。核糖体ITS1-5.8S-ITS2序列相似，不同之处在于每组的几个菌株中有两个单核苷酸交换。某些菌株的化学分类学分析确定了次级代谢产物生产中各组之间的差异。第一组的菌株具有北欧假单胞菌的化学型，而第二组的菌株具有疣状假单胞菌的化学型。RAPD和AFLP水平上的差异（与化学型差异平行）与最近产生producing曲霉毒素A的青霉菌重新分类为疣状假单胞菌或北欧假单胞菌一致。然而，ITS序列之间的同源性表明两种物种之间的系统发育关系。与最近将产och曲霉毒素A的青霉菌重新分类为疣状假单胞菌或野北假单胞菌一致。然而，ITS序列之间的同源性表明两种物种之间的系统发育关系。与最近将产och曲霉毒素A的青霉菌重新分类为疣状假单胞菌或野北假单胞菌一致。然而，ITS序列之间的同源性表明两种物种之间的系统发育关系。