BACKGROUND We have previously shown that UC blood (UCB) units can be volume-reduced manually, in a closed system, without major losses of nucleated and CD34(+) cells and without the addition of exogenous material. Our aim was to use an automated method for the separation of the UCB components using the Optipress II, extractor, with the 'buffy-coat' collection in a standardized volume. METHODS After centrifugation, the 51 UCB units were separated into the three blood components, plasma, buffy coat (BC) and red cells, using the Optipress II. The final volume of the BC fraction, rich in nucleated and progenitor CD34(+) cells, was set at 30 mL. The nucleated and CD34(+) cell content of the UCB collections and the resulting BC were evaluated. RESULTS The UCB units were grouped according to the volume collected: Group I < 80 mL and Group II > or = 80 mL. Standardization of the BC at 30 mL resulted in significant volume reduction for both groups, with median values of 51% in Group I and 70% in Group II. The nucleated and CD34(+) cell recoveries in the BC from Group I were 88% and 99% respectively; for Group II they were 80% and 97%. DISCUSSION This semi-automated method of volume reduction efficiently reduces low, as well as high volume UCB units, with good nucleated- and progenitor-cell yields. Being a closed system and free of external material, the risk of contamination is minimized. The resulting fractions are then available for validation studies of the unit, effectively fulfilling the main requisites for UCB banking.

中文翻译：

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使用 Optipress II 采血器处理脐带血


背景我们之前已经证明，UC 血液 (UCB) 单位可以在封闭系统中手动减容，不会造成有核细胞和 CD34(+) 细胞的大量损失，也无需添加外源物质。我们的目标是使用自动化方法通过 Optipress II 提取器分离 UCB 成分，并以标准化体积收集“血沉棕黄层”。方法 离心后，使用 Optipress II 将 51 个 UCB 单位分离成三种血液成分：血浆、血沉棕黄层 (BC) 和红细胞。富含有核细胞和祖细胞 CD34(+) 细胞的 BC 级分的最终体积设定为 30 mL。评估了 UCB 集合的有核细胞和 CD34(+) 细胞含量以及所得的 BC。结果 UCB 单位根据收集的体积进行分组：I 组 < 80 mL，II 组 > 或 = 80 mL。将 BC 标准化为 30 mL 后，两组的体积均显着减少，第一组的中值为 51%，第二组的中值为 70%。 I组BC中的有核细胞和CD34(+)细胞回收率分别为88%和99%；第二组分别为 80% 和 97%。讨论 这种半自动减容方法可有效减少低容量和高容量 UCB 单位，并具有良好的有核细胞和祖细胞产量。作为一个封闭的系统并且没有外部材料，污染的风险被最小化。所得分数可用于该装置的验证研究，有效满足 UCB 银行业务的主要要求。