Buchnericin-LB adsorbs to gram-positive but not to gram-negative bacteria. The tested gram-positive bacteria were species of Lactobacillus, Pediococcus, Leuconostoc, Enterococcus, Lactococcus, Listeria, Bacillus, Staphylococcus; gram-negative bacteria belonged to the genera Salmonella, Escherichia, Yersinia and Pseudomonas. Buchnericin-LB adsorption depended on pH but not on time and temperature. Also some anions of salts and lipoteichoic acid reduced or inhibited its adsorption. Treatment of cells and cell walls of sensitive bacteria with detergents, organic solvents or enzymes did not affect subsequent binding of buchnericin-LB. Treatment with buchnericin-LB caused sensitive cells to lose high amounts of intracellular K+ ions and UV-absorbing materials and became more permeable to o-nitrophenol-beta-D-galactopyranoside. Buchnericin-LB (640-2560 AU/ml) decreased the colony forming units (99%) and absorbance values of Listeria monocytogenes and Bacillus cereus. These results indicate that the mode of action of buchnericin-LB is bactericidal and its lethal effect is very rapid.

中文翻译：

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影响布氏霉素LB（布氏乳杆菌的校正）的细菌素buchnericin LB吸附的因素。

布氏霉素-LB吸附于革兰氏阳性细菌，但不吸附于革兰氏阴性细菌。所测试的革兰氏阳性细菌是乳杆菌，肺炎球菌，亮葡菌，肠球菌，乳球菌，李斯特菌，芽孢杆菌，葡萄球菌。革兰氏阴性细菌属于沙门氏菌，大肠埃希菌，耶尔森氏菌和假单胞菌属。布氏霉素-LB的吸附取决于pH值，而不取决于时间和温度。盐和脂磷壁酸的一些阴离子也降低或抑制了其吸附。用去污剂，有机溶剂或酶处理敏感细菌的细胞和细胞壁不会影响布氏霉素-LB的后续结合。用布氏霉素-LB处理会使敏感细胞损失大量细胞内K +离子和紫外线吸收材料，并使邻硝基苯酚-β-D-吡喃半乳糖苷更具渗透性。Buchnericin-LB（640-2560 AU / ml）降低了单核细胞增生李斯特菌和蜡状芽孢杆菌的菌落形成单位（99％）和吸光度值。这些结果表明布氏霉素-LB的作用方式是杀菌的，其致死作用非常迅速。