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The mechanism of action of antioxidants against lipoprotein peroxidation, evaluation based on kinetic experiments.
Progress in Lipid Research ( IF 14.0 ) Pub Date : 2002-04-18 , DOI: 10.1016/s0163-7827(01)00026-1
Ilya Pinchuk 1 , Dov Lichtenberg
Affiliation  

Peroxidation of blood lipoproteins is regarded as a key event in the development of atherosclerosis. Hence, attenuation of the oxidative modification of lipoproteins by natural and synthetic antioxidants in vivo is considered a possible way of prevention of cardiovascular disorders. The assessment of the susceptibility of lipoproteins to oxidation is commonly based on in vitro oxidation experiments. Monitoring of oxidation provides the kinetic profile characteristic for the given lipoprotein preparation. The kinetic profile of peroxidation is characterized by three major parameters: the lag preceding rapid oxidation, the maximal rate of oxidation (V(max)) and the maximal accumulation of oxidation products (OD(max)). Addition of antioxidants alters this pattern, affecting the kinetic parameters of oxidation. In particular, antioxidants may prolong the lag and/or decrease the V(max) and/or decrease the OD(max). Such specific variation of the set of kinetic parameters may provide important information on the mechanism of the inhibitory action of a given antioxidant (scavenging free radicals, metal-binding or other mechanisms). Numerous natural and synthetic compounds were reported to inhibit oxidation of lipoproteins. Based on the analysis of reported effects and theoretical considerations, we propose a simple protocol that relates the kinetic effects of a given antioxidant to the mechanism of its action.

中文翻译:

抗氧化剂抗脂蛋白过氧化作用的机理,根据动力学实验进行评估。

血液脂蛋白的过氧化被认为是动脉粥样硬化发展的关键事件。因此,认为天然和合成抗氧化剂在体内减弱脂蛋白的氧化修饰是预防心血管疾病的可能方法。脂蛋白对氧化敏感性的评估通常基于体外氧化实验。氧化的监测为给定的脂蛋白制剂提供了动力学特征。过氧化的动力学特征由三个主要参数表征:快速氧化之前的滞后,最大氧化速率(V(max))和最大氧化积累量(OD(max))。抗氧化剂的添加改变了这种模式,影响了氧化动力学参数。特别是,抗氧化剂可能会延长延迟和/或降低V(max)和/或降低OD(max)。一组动力学参数的这种特定变化可以提供有关给定抗氧化剂的抑制作用机理(清除自由基,金属结合或其他机理)的重要信息。据报道,许多天然和合成的化合物都能抑制脂蛋白的氧化。基于对所报告作用的分析和理论考虑,我们提出了一种简单的方案,该方案将给定抗氧化剂的动力学作用与其作用机理相关联。金属绑定或其他机制)。据报道,许多天然和合成的化合物都能抑制脂蛋白的氧化。基于对所报告作用的分析和理论考虑,我们提出了一种简单的方案,该方案将给定抗氧化剂的动力学作用与其作用机理相关联。金属绑定或其他机制)。据报道,许多天然和合成的化合物都能抑制脂蛋白的氧化。基于对所报告作用的分析和理论考虑,我们提出了一种简单的方案,将给定抗氧化剂的动力学作用与其作用机理相关联。
更新日期:2019-11-01
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