Platelets exert a crucial function in haemostasis, wound repair, and the formation of vascular plugs, underlying thrombotic diseases such as stroke and myocardial infarction. Analysis of platelet biochemistry is largely dependent on protein analysis as platelets are anucleated cells providing little analytical target for DNA or RNA based strategies. Here we present data from our analysis of the human platelet proteome, the entire set of proteins building a platelet at a given point in time. Proteins were separated by two-dimensional electrophoresis (2-DE) using broad and narrow range pH gradients in the isoelectric focusing step. Consequently, a high-resolution 2-DE proteome map has been generated that comprises approximately 2300 different protein features. From the 536 protein features detected in the 4-5 pI range 284 features were identified by electrospray ionisation time of flight tandem mass spectrometry. These 284 proteins originate from 123 different open reading frames. This includes the five human proteins KIAA0193, KIAA0573, KIAA0830, WUGSC:H_DJ0777O23 protein, and cytokine receptor related protein 4, all isolated for the first time. The data are discussed with regard to proteome characteristics, protein function, and the high prevalence of signalling molecules. This study contributes to a more thorough and holistic understanding of platelet biology, helping to build the basis for future identification of new drug targets and therapeutic strategies.

中文翻译：

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全面分析血小板蛋白质组。

血小板在止血，伤口修复和血管栓塞的形成中起着至关重要的作用，而血栓形成是潜在的血栓性疾病，例如中风和心肌梗塞。血小板生化分析很大程度上取决于蛋白质分析，因为血小板是无核细胞，几乎没有为基于DNA或RNA的策略提供分析目标。在这里，我们介绍了人类血小板蛋白质组的分析数据，即在给定时间点构建血小板的整套蛋白质。在等电聚焦步骤中使用宽范围和窄范围的pH梯度通过二维电泳（2-DE）分离蛋白质。因此，已经产生了高分辨率的2-DE蛋白质组图，其包含大约2300个不同的蛋白质特征。通过在4-5 pI范围内检测到的536种蛋白质特征，通过电喷雾电离飞行时间串联质谱法鉴定了284种特征。这284种蛋白质来自123个不同的开放阅读框。这包括五个人类蛋白质KIAA0193，KIAA0573，KIAA0830，WUGSC：H_DJ0777O23蛋白和细胞因子受体相关蛋白4，它们都是首次分离。讨论了有关蛋白质组学特征，蛋白质功能和信号分子高发生率的数据。这项研究有助于更全面和全面地了解血小板生物学，有助于为将来确定新药靶标和治疗策略奠定基础。这包括五个人类蛋白质KIAA0193，KIAA0573，KIAA0830，WUGSC：H_DJ0777O23蛋白和细胞因子受体相关蛋白4，它们都是首次分离。讨论了有关蛋白质组学特征，蛋白质功能和信号分子高发生率的数据。这项研究有助于更全面和全面地了解血小板生物学，有助于为将来确定新药靶标和治疗策略奠定基础。这包括五个人类蛋白质KIAA0193，KIAA0573，KIAA0830，WUGSC：H_DJ0777O23蛋白和细胞因子受体相关蛋白4，它们都是首次分离。讨论了有关蛋白质组学特征，蛋白质功能和信号分子高发生率的数据。这项研究有助于更全面和全面地了解血小板生物学，有助于为将来确定新药靶标和治疗策略奠定基础。