The perchlorate (ClO4(-))-respiring organism, strain perclace, can grow using nitrate (NO3(-)) as a terminal electron acceptor. In resting cell suspensions, NO(-) grown cells reduced ClO4(-), and ClO4(-) grown cells reduced NO3(-). Activity assays showed that nitrate reductase (NR) activity was 1.31 micromol min(-1) (mg protein)-1 in (ClO4)- grown cells, and perchlorate reductase (PR) activity was 4.24 micromol min(-1) (mg protein)(-1) in NO3(-) grown cells. PR activity was detected within the periplasmic space, with activities as high as 14 pmol min(-1) (mg protein)(-1). The NR had a pH optimum of 9.0 while the PR had an optimum of 8.0. This study suggests that separate terminal reductases are present in strain perclace to reduce NO3(-) and ClO4(-).

中文翻译：

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高氯酸盐呼吸细菌中的高氯酸盐和硝酸盐还原酶活性。

可以吸收高氯酸盐（ClO4（-））的有机体菌株Perclace，可以使用硝酸盐（NO3（-））作为末端电子受体来生长。在静止的细胞悬液中，NO（-）生长的细胞还原ClO4（-），而ClO4（-）生长的细胞还原NO3（-）。活性测定表明，在（ClO4）生长的细胞中硝酸盐还原酶（NR）活性为1.31 micromol min（-1）（mg蛋白）-1，高氯酸盐还原酶（PR）活性为4.24 micromol min（-1）（mg蛋白）-1 ）（-1）在NO3（-）生长的细胞中。在周质空间内检测到PR活性，其活性高达14 pmol min（-1）（mg蛋白）（-1）。NR的最适pH为9.0，而PR的最适pH为8.0。这项研究表明，菌株perclace中存在单独的末端还原酶，以还原NO3（-）和ClO4（-）。