The present study has investigated the efficiency of 0.9% saline solution and Braun-Collins solution in the preservation of sheep preantral follicles in situ, at different temperatures and different incubation periods. The ovaries were divided into 19 fragments for each animal. One ovarian fragment was taken randomly and immediately fixed for histology (control-time zero). The other 18 ovarian fragments were randomly distributed in tubes containing 2ml of 0.9% saline solution or Braun-Collins solution at 4, 20 or 39 degrees C for 4, 12 or 24h. A total of 1629, 208 and 63 primordial, primary and secondary follicles were examined, respectively. Histological analysis showed that the storage of ovarian fragments in both solutions at 20 or 39 degrees C significantly reduced the percentage of morphologically normal preantral follicles (MNPF) when compared with the control, except after preservation in 0.9% saline solution at 20 degrees C for 4h. In contrast, the preservation at 4 degrees C, in both solutions, kept the percentage of MNPF similar to control values. In conclusion, this study shows for the first time that sheep preantral follicles can be preserved in situ successfully at 4 degrees C in 0.9% saline solution or Braun-Collins solution for up to 24h.

中文翻译：

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羊腔前卵泡在 0.9% 生理盐水和 Braun-Collins 溶液中的短期原位维持

本研究调查了 0.9% 盐水溶液和 Braun-Collins 溶液在不同温度和不同潜伏期下原位保存绵羊窦前卵泡的效率。每只动物的卵巢被分成19个片段。随机取出一个卵巢碎片并立即固定用于组织学（控制时间为零）。其他 18 个卵巢碎片随机分布在含有 2ml 0.9% 盐水溶液或 Braun-Collins 溶液的管中，在 4、20 或 39 摄氏度下放置 4、12 或 24 小时。总共分别检查了 1629、208 和 63 个原始、初级和次级卵泡。组织学分析表明，与对照相比，在 20 或 39 摄氏度的两种溶液中储存卵巢碎片显着降低了形态正常的窦前卵泡 (MNPF) 的百分比，但在 20 摄氏度的 0.9% 盐水溶液中保存 4 小时后除外. 相比之下，在 4 摄氏度的保存，在这两种解决方案中，使 MNPF 的百分比与对照值相似。总之，这项研究首次表明，绵羊窦前卵泡可以在 4 摄氏度的 0.9% 盐水溶液或布劳恩-柯林斯溶液中成功原位保存长达 24 小时。