We investigated the protective effect of alpha lipoic acid (ALA) against cisplatin (CIS) induced hepatotoxicity in rats. ALA is an antioxidant and anti-inflammatory agent that exhibits free radical scavenger properties and direct antioxidant effects on recycling of other cellular antioxidants. We used four equal groups of rats. The control group: saline solution (0.9%) was administered intraperitoneally (i.p.); ALA group: administered a single dose 100 mg/kg ALA i.p. for 10 days; CIS group was administered a single dose 5 mg/kg CIS i.p. on the first day of the study; CIS + ALA group was administered a single dose 5 mg/kg CIS i.p. on the first day of study, then 100 mg/kg ALA i.p. for 10 days. In the CIS group, Bax, caspase3, malondialdehyde (MDA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were increased, whereas Bcl-2, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) levels were decreased compared to the control group. In the CIS + ALA group, Bax, caspase 3, MDA, AST and ALT levels were decreased, whereas Bcl-2, SOD, CAT and GPx levels were increased compared to the CIS group. In the CIS group we found intense perivenule sinusoid dilation, karyomegaly, pyknotic and karyolytic cells, central vein congestion, parenchymal inflammation, mild bile duct proliferation and periportal sinusoid dilation. Histological liver damage was reduced in the CIS + ALA group. ALA may useful for treating CIS induced hepatotoxicity owing to its antioxidant and anti-inflammatory effects.

中文翻译：

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α硫辛酸对顺铂诱导的大鼠肝毒性的保护作用。

我们调查了α硫辛酸（ALA）对顺铂（CIS）诱导的大鼠肝毒性的保护作用。ALA是一种抗氧化剂和消炎剂，具有自由基清除剂的特性，并在其他细胞抗氧化剂的循环利用方面具有直接的抗氧化剂作用。我们使用了四组相等的大鼠。对照组：腹膜内（ip）给予盐溶液（0.9％）；ALA组：单剂量100 mg / kg ALA腹膜内给药10天；在研究的第一天，给CIS组腹膜内注射单剂量5 mg / kg CIS；在研究的第一天，对CIS + ALA组进行单剂量5 mg / kg CIS腹膜内给药，然后100 ip / kg ALA腹膜内给药10天。在CIS组中，Bax，caspase3，丙二醛（MDA），天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）的水平升高，与对照组相比，Bcl-2，超氧化物歧化酶（SOD），过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）水平降低。与CIS组相比，CIS + ALA组的Bax，caspase 3，MDA，AST和ALT降低，而Bcl-2，SOD，CAT和GPx升高。在CIS组中，我们发现了强烈的小静脉窦正弦扩张，核仁肿大，解热和溶核细胞，中心静脉充血，实质性炎症，轻度胆管增生和门静脉窦窦扩张。CIS + ALA组肝脏组织学损伤减少。由于其抗氧化剂和抗炎作用，ALA可用于治疗CIS诱导的肝毒性。与CIS组相比，Bax，caspase 3，MDA，AST和ALT水平降低，而Bcl-2，SOD，CAT和GPx水平升高。在CIS组中，我们发现了强烈的小静脉窦正弦扩张，核仁肿大，解热和溶核细胞，中心静脉充血，实质性炎症，轻度胆管增生和门静脉窦窦扩张。CIS + ALA组肝脏组织学损伤减少。由于其抗氧化剂和抗炎作用，ALA可用于治疗CIS诱导的肝毒性。与CIS组相比，Bax，caspase 3，MDA，AST和ALT水平降低，而Bcl-2，SOD，CAT和GPx水平升高。在CIS组中，我们发现了强烈的小静脉窦正弦扩张，核仁肿大，解热和溶核细胞，中心静脉充血，实质性炎症，轻度胆管增生和门静脉窦窦扩张。CIS + ALA组肝脏组织学损伤减少。由于其抗氧化剂和抗炎作用，ALA可用于治疗CIS诱导的肝毒性。轻度胆管增生和门静脉窦窦扩张。CIS + ALA组的肝脏组织学损伤减少。由于其抗氧化剂和抗炎作用，ALA可用于治疗CIS诱导的肝毒性。轻度胆管增生和门静脉窦窦扩张。CIS + ALA组肝脏组织学损伤减少。由于其抗氧化剂和抗炎作用，ALA可用于治疗CIS诱导的肝毒性。