Excessive use of antibiotics contributes to the selection of resistant bacteria and intestinal colonization with multiresistant pathogens poses a risk factor for subsequent infections. The present study assessed vancomycin-resistant enterococci (VRE) carriage rates in patients admitted to our tertiary care hospital. Stool samples sent for routine culturing were screened with vancomycin containing solid or broth enrichment media. VRE isolates were identified with matrix-assisted laser desorption/ionization-time of flight mass spectrometry and antibiotic susceptibilities were tested by E-test. Vancomycin resistance genes were detected by polymerase chain reaction. Medical records of carriers were examined for suspected risk factors for colonization. Altogether 3025 stool specimens were analyzed. Solid media identified a VRE carriage rate of 2.2% while broth enrichment detected 5.8%. Seventy percent of the isolates were Enterococcus faecium. VanB genotype was detected in 38.2%, VanA in 37.3%, VanC1 in 22.6%, and VanC2 in 1.9%. All VRE were sensitive to linezolid, daptomycin, and tigecycline. Collective risk factors for carriage were diabetes, normal flora absence, Clostridioides difficile positivity, longer hospital stay, and advanced age. 78.5% of the carriers received antibiotic therapy which was metronidazole in most cases (47.3%). We recommend regular screening of risk groups such as patients with diabetes, history of recent hospitalization, or former C. difficile infection as an imperative step for preventing VRE dissemination.

中文翻译：

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在匈牙利的一家三级医院评估耐万古霉素肠球菌（VRE）的肠道运输率。

过量使用抗生素有助于耐药菌的选择，多耐药病原菌在肠道中的定殖是随后感染的危险因素。本研究评估了入住我们三级医院的患者对万古霉素耐药的肠球菌（VRE）携带率。用含有固体或肉汤富集培养基的万古霉素筛选送去常规培养的粪便样品。用基质辅助激光解吸/电离飞行时间质谱仪鉴定了VRE分离物，并通过E检验测试了抗生素敏感性。通过聚合酶链反应检测万古霉素抗性基因。检查了携带者的病历，以确定是否有定植的危险因素。总共分析了3025个粪便标本。固态媒体确定的VRE承载率为2。2％，而肉汤浓缩检测为5.8％。分离株的百分之七十是粪肠球菌。检测到VanB基因型的占38.2％，检测到VanA的占37.3％，检测到VanC1的占22.6％，检测到VanC2的基因占1.9％。所有VRE对利奈唑胺，达托霉素和替加环素敏感。携带的集体危险因素为糖尿病，正常菌群缺失，艰难梭菌阳性，住院时间较长和年龄增长。78.5％的携带者接受了抗生素治疗，大多数情况下是甲硝唑（47.3％）。我们建议定期筛查危险人群，例如糖尿病患者，近期住院病史或以前的艰难梭菌感染，作为预防VRE传播的必要步骤。6％，而VanC2为1.9％。所有VRE对利奈唑胺，达托霉素和替加环素敏感。携带的集体危险因素为糖尿病，正常菌群缺失，艰难梭菌阳性，住院时间较长和年龄增长。78.5％的携带者接受了抗生素治疗，大多数情况下是甲硝唑（47.3％）。我们建议定期筛查危险人群，例如糖尿病患者，近期住院病史或以前的艰难梭菌感染，作为预防VRE传播的必要步骤。6％，而VanC2为1.9％。所有VRE对利奈唑胺，达托霉素和替加环素敏感。携带的集体危险因素为糖尿病，正常菌群缺失，艰难梭菌阳性，住院时间较长和年龄增长。78.5％的携带者接受了抗生素治疗，大多数情况下是甲硝唑（47.3％）。我们建议定期筛查危险人群，例如糖尿病患者，近期住院病史或以前的艰难梭菌感染，作为预防VRE传播的必要步骤。5％的携带者接受了抗生素治疗，在大多数情况下为甲硝唑（47.3％）。我们建议定期筛查危险人群，例如糖尿病患者，近期住院病史或以前的艰难梭菌感染，作为预防VRE传播的必要步骤。5％的携带者接受了抗生素治疗，在大多数情况下为甲硝唑（47.3％）。我们建议定期筛查危险人群，例如糖尿病患者，近期住院病史或以前的艰难梭菌感染，作为预防VRE传播的必要步骤。