Nppa is a cardiac hormone which plays critical roles in regulating salt–water balance. Its expression is restricted to the atria of the healthy post‐natal heart. During heart development, spatio‐temporal expression of Nppa is dynamically changed within the heart and becomes restricted to the atria upon birth. In contrast to its atrial specific expression after birth, Nppa is re‐expressed in the adult ventricles in response to cardiac hypertrophy. To study cardiac chamber specification during development and pathological cardiac remodeling during heart disease, we generated a novel Nppa reporter mouse line by knocking‐in a tagBFP reporter cassette into 3′‐UTR of the Nppa gene without disrupting the endogenous gene. Our results demonstrated dynamic tagBFP expression in the developing heart, recapitulating the spatiotemporal expression pattern of endogenous Nppa. We also found that Nppa‐tagBFP is induced in the ventricle during pathological remodeling. Taken together, Nppa‐tagBFP reporter knock‐in mouse model described in this article will serve as a valuable tool to study cardiac chamber specification during development as well as pathological cardiac remodeling.

中文翻译：

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Nppa-tagBFP报告基因敲入小鼠系的生成，用于研究心室规格。

Nppa是一种心脏激素，在调节盐水平衡中起着至关重要的作用。它的表达仅限于健康的产后心脏的心房。在心脏发育期间，Nppa的时空表达会在心脏内动态变化，并在出生时局限于心房。与出生后其心房特异性表达相反，Nppa在心脏肥大后在成人心室中重新表达。为了研究心脏疾病中的发展和病理性心脏重构过程中心脏室规范，我们产生了一种新NPPA通过敲入记者鼠标线tagBFP将报告盒插入Nppa基因的3'-UTR，而不会破坏内源基因。我们的结果证明了动态tagBFP在发育中的心脏中表达，概括了内源性Nppa的时空表达模式。我们还发现，在病理重塑期间，Nppa-tagBFP在心室中被诱导。综上所述，本文所述的Nppa-tagBFP报告基因敲入小鼠模型将成为研究发育过程中心脏腔室规格以及病理性心脏重塑的有价值的工具。