当前位置: X-MOL 学术J. Plant Growth. Regul. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The role of calcium in growth induced by indole-3-acetic acid and gravity in the leaf-sheath pulvinus of oat (Avena sativa)
Journal of Plant Growth Regulation ( IF 3.9 ) Pub Date : 1992-04-01 , DOI: 10.1007/bf00198021
T G Brock 1 , J Burg , N S Ghosheh , P B Kaufman
Affiliation  

Leaf-sheath pulvini of excised segments from oat (Avena sativa L.) were induced to grow by treatment with 10 μM indole-3-acetic acid (IAA), gravistimulation, or both, and the effects of calcium, EGTA, and calcium channel blockers on growth were evaluated. Unilaterally applied calcium (10 mM CaCl2) significantly inhibited IAA-induced growth in upright pulvini but had no effect on growth induced by either gravity or gravity plus IAA. Calcium alone had no effect on upright pulvini. The calcium chelator EGTA alone (10 mM) stimulated growth in upright pulvini. However, EGTA had no effect on either IAA-or gravity-induced growth but slightly diminished growth in IAA-treated gravistimulated pulvini. The calcium channel blockers lanthanum chloride (25 mM), verapamil (2.5 mM), and nifedipine (2.5 mM) greatly inhibited growth as induced by IAA (⩾50% inhibition) or IAA plus gravity (20% inhibition) but had no effect on gravistimulated pulvini. Combinations of channel blockers were similar in effect on IAA action as individual blockers. Since neither calcium ions nor EGTA significantly affected the graviresponse of pulvini, we conclude that apoplastic calcium is unimportant in leaf-sheath pulvinus gravitropism. The observation that calcium ions and calcium channel blockers inhibit IAA-induced growth, but have no effect on gravistimulated pulvini, further supports previous observations that gravistimulation alters the responsiveness of pulvini to IAA.

中文翻译:

钙在由吲哚-3-乙酸和重力诱导的燕麦叶鞘生长中的作用(Avena sativa)

通过用 10 μM 吲哚-3-乙酸 (IAA)、重力刺激或两者处理以及钙、EGTA 和钙通道的作用,诱导燕麦 (Avena sativa L.) 切下片段的叶鞘生长评估生长阻滞剂。单方面应用钙 (10 mM CaCl2) 显着抑制 IAA 诱导的直立 pulvini 生长,但对重力或重力加 IAA 诱导的生长没有影响。单独的钙对直立的 pulvini 没有影响。单独的钙螯合剂 EGTA (10 mM) 刺激直立 pulvini 的生长。然而,EGTA 对 IAA 或重力诱导的生长没有影响,但对 IAA 处理的重力刺激的 pulvini 的生长略有减弱。钙通道阻滞剂氯化镧 (25 mM)、维拉帕米 (2.5 mM) 和硝苯地平 (2. 5 mM)极大地抑制了由 IAA(⩾ 50% 抑制)或 IAA 加重力(20% 抑制)诱导的生长,但对重力刺激的 pulvini 没有影响。通道阻滞剂的组合对 IAA 作用的影响与单个阻滞剂相似。由于钙离子和 EGTA 都没有显着影响 pulvini 的重力反应,我们得出结论,质外体钙在叶鞘 pulvinus 向重力性中并不重要。钙离子和钙通道阻滞剂抑制 IAA 诱导的生长,但对重力刺激的 pulvini 没有影响的观察结果进一步支持了先前的观察,即重力刺激改变了 pulvini 对 IAA 的反应性。由于钙离子和 EGTA 都没有显着影响 pulvini 的重力反应,我们得出结论,质外体钙在叶鞘 pulvinus 向重力性中并不重要。钙离子和钙通道阻滞剂抑制 IAA 诱导的生长,但对重力刺激的 pulvini 没有影响的观察结果进一步支持了先前的观察,即重力刺激改变了 pulvini 对 IAA 的反应性。由于钙离子和 EGTA 都没有显着影响 pulvini 的重力反应,我们得出结论,质外体钙在叶鞘 pulvinus 向重力性中并不重要。钙离子和钙通道阻滞剂抑制 IAA 诱导的生长,但对重力刺激的 pulvini 没有影响的观察结果进一步支持了先前的观察,即重力刺激改变了 pulvini 对 IAA 的反应性。
更新日期:1992-04-01
down
wechat
bug