A method to use sequential rounds of immunofluorescent labeling in cell cultures is presented. The method is based on the utilization of a non-liquid reducing agent, sodium dithionite, in conjunction with ionic or non-ionic detergents (SDS or TX100, respectively) at room temperature. This method preserves cell morphology and substrate antigenicity, and operates through the complete extraction of most primary and secondary antibodies. Using this protocol, the sequential immunolocalization of different proteins is possible, without signal interference with previous immunolabeling rounds. In addition, the method is also useful to recycle blotted membranes in immunoblots.

中文翻译：

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回收培养的细胞进行免疫荧光标记。

提出了在细胞培养物中使用连续几轮免疫荧光标记的方法。该方法基于在室温下结合离子或非离子去污剂（分别为SDS或TX100）使用非液体还原剂连二亚硫酸钠。该方法保留了细胞形态和底物抗原性，并通过完全提取大多数一抗和二抗而起作用。使用此协议，可以对不同蛋白质进行顺序免疫定位，而不会干扰先前的免疫标记轮次。此外，该方法还可用于回收免疫印迹中的印迹膜。