Goat ovarian preantral follicles were morphologically and ultrastructurally described in this work. Primordial follicles are oocytes surrounded by one layer of squamous or squamous-cuboidal granulosa cells; primary follicles have a single layer of cuboidal granulosa cells, and secondary follicles are oocytes surrounded by two or more layers of cuboidal granulosa cells. At all developmental stages a thick layer of glycoproteins, the basement membrane, surrounded the preantral follicles. The quiescent oocyte is spherical or oval and it has a large eccentrically located nucleus with a conspicuous nucleolus. The organelles were uniformly distributed in the cytoplasm. A large number of vesicles were spread throughout the cytoplasm in all the oocytes. The cytoplasm of oocytes also contains numerous rounded mitochondria besides the usual organelles. As the follicle develops, the mitochondria become elongated. The communication between the oocyte and the granulosa cells is apparently mediated through endocytosis as indicated by the abundant coated pits and vesicles noted in the cortical cytoplasm of the oocyte. The oocyte plasma membrane presented projections that penetrated between adjacent granulosa cells and a few short microvilli lying parallel to the oocyte surface. In secondary follicles, patches of zona pellucida material were observed. Overall, the results indicate that the morphological and ultrastructural organization of caprine preantral follicles resembles that of other mammals. However, some particularities were observed, and that may indicate species specific differences.

中文翻译：

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山羊窦前卵泡的光学显微镜和超微结构表征

在这项工作中对山羊卵巢腔前卵泡进行了形态学和超微结构描述。原始卵泡是被一层鳞状或鳞状立方颗粒细胞包围的卵母细胞；初级卵泡具有单层立方颗粒细胞，次级卵泡是被两层或多层立方颗粒细胞包围的卵母细胞。在所有发育阶段，一层厚厚的糖蛋白，即基底膜，包围着窦前卵泡。静止的卵母细胞呈球形或椭圆形，有一个大的偏心核和明显的核仁。细胞器均匀分布在细胞质中。在所有卵母细胞中，大量囊泡遍布整个细胞质。除了通常的细胞器外，卵母细胞的细胞质还含有许多圆形的线粒体。随着卵泡的发育，线粒体被拉长。卵母细胞和颗粒细胞之间的交流显然是通过内吞作用介导的，如卵母细胞皮质细胞质中发现的大量有涂层的凹坑和囊泡所示。卵母细胞质膜呈现穿透相邻颗粒细胞和一些与卵母细胞表面平行的短微绒毛之间的突起。在次级卵泡中，观察到透明带物质的斑块。总体而言，结果表明山羊窦前卵泡的形态和超微结构组织类似于其他哺乳动物。然而，观察到了一些特殊性，这可能表明物种特定的差异。卵母细胞和颗粒细胞之间的交流显然是通过内吞作用介导的，如卵母细胞皮质细胞质中发现的大量有涂层的凹坑和囊泡所示。卵母细胞质膜呈现穿透相邻颗粒细胞和一些与卵母细胞表面平行的短微绒毛之间的突起。在次级卵泡中，观察到透明带物质的斑块。总体而言，结果表明山羊窦前卵泡的形态和超微结构组织类似于其他哺乳动物。然而，观察到了一些特殊性，这可能表明物种特定的差异。卵母细胞和颗粒细胞之间的交流显然是通过内吞作用介导的，如卵母细胞皮质细胞质中发现的大量有涂层的凹坑和囊泡所示。卵母细胞质膜呈现穿透相邻颗粒细胞和一些与卵母细胞表面平行的短微绒毛之间的突起。在次级卵泡中，观察到透明带物质的斑块。总体而言，结果表明山羊窦前卵泡的形态和超微结构组织类似于其他哺乳动物。然而，观察到了一些特殊性，这可能表明物种特定的差异。卵母细胞质膜呈现穿透相邻颗粒细胞和一些与卵母细胞表面平行的短微绒毛之间的突起。在次级卵泡中，观察到透明带物质的斑块。总体而言，结果表明山羊窦前卵泡的形态和超微结构组织类似于其他哺乳动物。然而，观察到了一些特殊性，这可能表明物种特定的差异。卵母细胞质膜呈现穿透相邻颗粒细胞和一些与卵母细胞表面平行的短微绒毛之间的突起。在次级卵泡中，观察到透明带物质的斑块。总体而言，结果表明山羊窦前卵泡的形态和超微结构组织类似于其他哺乳动物。然而，观察到了一些特殊性，这可能表明物种特定的差异。