The capacity of the microbes to reduce the metal has been demonstrated. The immobilised induced microbes with toxic chemical CuCl(2) was used to reduce the Cu ions as elemental metal and by using the response surface methodology the parameters such as the inducer concentration, the time of inducer addition which are concerned with the growth and formation of specific enzymes and the initial substrate concentration, initial pH of the substrate solution and the time of reaction which are concerned with the biocatalytic reduction of the metal ions were optimised for maximum reduction. The elemental copper reduced and removed experimentally from its ionic state at the optimum conditions was 54.82ppm.

中文翻译：

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通过在分解代谢抑制条件下生长的酿酒酵母粗酶裂解物对铜离子生物催化还原参数的化学计量学优化

微生物还原金属的能力已被证明。使用有毒化学物质 CuCl(2) 固定化诱导微生物还原作为元素金属的 Cu 离子，并通过使用响应面方法确定诱导剂浓度、诱导剂添加时间等参数，这些参数与铜离子的生长和形成有关。优化了特定酶和初始底物浓度、底物溶液的初始 pH 值和与金属离子生物催化还原有关的反应时间，以实现最大还原。在最佳条件下通过实验从其离子态还原和去除的元素铜为 54.82ppm。