BACKGROUND Disturbance in calcium metabolism has been suggested in the pathogenesis of hypertension, however, membrane calcium content in humans has not been studied in detail yet in primary hypertension. We compared plasma, intracellular and membrane calcium concentrations in erythrocytes of patients with essential hypertension and in healthy, normotensive control subjects to determine a possible alteration of membrane calcium in primary hypertension. SUBJECTS AND METHODS Thirty-four never treated patients with essential hypertension were included and 34 healthy, age- and sex-matched volunteers served as controls. Atomic absorption spectroscopy was used for measurement of intracellular and membrane calcium content in erythrocytes and plasmalemmal preparations. RESULTS Plasma and intracellular Ca(++) concentrations were not significantly different between hypertensives and controls (plasma: 2.59 +/- 0.18 vs2.50 +/- 0.16 mmol/l, intracellular: 1.89 +/- 0.20 mmol/l vs 1.97 +/- 0.24 mmol/l, NS resp., mean +/- s.e.m.). However, membrane calcium content was significantly higher in hypertensive patients compared to control subjects (2.38 +/- 0.28 micromol/g membraneous protein vs0.86 +/- 0.32 micromol/g membrane protein, P < 0.01). Membrane calcium content was correlated to mean arterial blood pressure (r = 0.59, P < 0.01). CONCLUSION Membrane calcium content is significantly increased in patients with untreated primary hypertension and correlates to blood pressure levels. This data suggest, that an membrane mechanism may contribute to alterations in calcium metabolism and to the pathogenesis of primary hypertension.

中文翻译：

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原发性高血压中膜钙离子浓度升高：与发病机制有因果关系吗？

背景技术已经在高血压的发病机理中暗示了钙代谢的紊乱，但是，在原发性高血压中尚未详细研究人的膜钙含量。我们比较了原发性高血压患者和健康，正常血压控制对象的红细胞中血浆，细胞内和膜中钙的浓度，以确定原发性高血压中膜钙的可能改变。受试者和方法纳入了34例从未接受过治疗的原发性高血压患者，并以34名健康，年龄和性别相匹配的志愿者作为对照。原子吸收光谱法用于测量红细胞和血浆制品中细胞内和膜中钙的含量。结果高血压和对照组之间血浆和细胞内Ca（++）浓度无显着差异（血浆：2.59 +/- 0.18 vs2.50 +/- 0.16 mmol / l，细胞内：1.89 +/- 0.20 mmol / l vs 1.97 + +/- 0.24 mmol / l，NS响应，平均值+/- sem）。然而，与对照组相比，高血压患者的膜钙含量明显更高（2.38 +/- 0.28 micromol / g膜蛋白对0.86 +/- 0.32 micromol / g膜蛋白，P <0.01）。膜钙含量与平均动脉压相关（r = 0.59，P <0.01）。结论未经治疗的原发性高血压患者的膜钙含量显着增加，并且与血压水平相关。这些数据表明，