Salinity and drought are the major abiotic stresses that limit agricultural productivity. Application of plant growth promoting rhizobacteria (PGPR) is an attractive technology but with the bottlenecks of reduced efficacy and survivability in the environment. For increased efficiency of PGPR strains, the impact of stresses on the native bacterial community needs to be studied. Experimentally induced stresses would be ideal to assess the immediate perturbances in the structure of soil bacterial community. Hence, the study focused on the effect of experimentally‐induced salinity, and drought stress on rhizospheric bacterial community of Cajanus cajan. A plant growth experiment was set up to induce salinity and drought stresses. Shifts in the bacterial community were assessed by a culture‐independent technique of denaturing gradient gel electrophoresis using 16S ribosomal RNA gene and transcript as markers, leading to a comparison of the resident with the active bacterial community. The impact on plant was evaluated by measurement of plant biometrics. Further, salinity and drought‐stressed conditions led to distinct shifts in native and active rhizospheric bacterial community, corresponding to the higher decline at induction of stresses, and stabilization at later time points. The study encompasses the perturbations in the active and resident rhizospheric bacterial community caused by the induction of two different abiotic stresses along the plant's growth.

中文翻译：

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非生物应激源对 Cajanus cajan 原生根际细菌群落的影响

盐分和干旱是限制农业生产力的主要非生物胁迫。植物生长促进根际细菌 (PGPR) 的应用是一项有吸引力的技术，但其在环境中的功效和生存能力降低的瓶颈。为了提高 PGPR 菌株的效率，需要研究压力对天然细菌群落的影响。实验诱导的压力对于评估土壤细菌群落结构的直接扰动是理想的。因此，该研究的重点是实验诱导的盐度和干旱胁迫对 Cajanus cajan 根际细菌群落的影响。建立植物生长实验以诱导盐分和干旱胁迫。通过使用 16S 核糖体 RNA 基因和转录本作为标记的变性梯度凝胶电泳的不依赖培养的技术评估细菌群落的变化，从而将居民与活跃的细菌群落进行比较。通过测量植物生物特征来评估对植物的影响。此外，盐度和干旱胁迫条件导致原生和活跃的根际细菌群落发生明显变化，对应于诱导胁迫时下降幅度更大，并在以后的时间点稳定。该研究包括在植物生长过程中诱导两种不同的非生物胁迫引起的活跃和常驻根际细菌群落的扰动。导致将居民与活跃的细菌群落进行比较。通过测量植物生物特征来评估对植物的影响。此外，盐度和干旱胁迫条件导致原生和活跃的根际细菌群落发生明显变化，对应于诱导胁迫时下降幅度更大，并在以后的时间点稳定。该研究包括在植物生长过程中诱导两种不同的非生物胁迫引起的活跃和常驻根际细菌群落的扰动。导致将居民与活跃的细菌群落进行比较。通过测量植物生物特征来评估对植物的影响。此外，盐度和干旱胁迫条件导致原生和活跃的根际细菌群落发生明显变化，对应于诱导胁迫时下降幅度更大，并在以后的时间点稳定。该研究包括在植物生长过程中诱导两种不同的非生物胁迫引起的活跃和常驻根际细菌群落的扰动。并在以后的时间点稳定。该研究包括在植物生长过程中诱导两种不同的非生物胁迫引起的活跃和常驻根际细菌群落的扰动。并在以后的时间点稳定。该研究包括在植物生长过程中诱导两种不同的非生物胁迫引起的活跃和常驻根际细菌群落的扰动。