Long noncoding (lnc)RNAs have been implicated in the development and progression of atherosclerosis. However, the expression and mechanism of action of lncRNAs in atherosclerosis are still unclear. We implemented microarray analysis in human advanced atherosclerotic plaques and normal arterial intimae to detect the lncRNA and mRNA expression profile. Gene Ontology functional enrichment and pathway analyses were applied to explore the potential functions and pathways involved in the pathogenesis of atherosclerosis. A total of 236 lncRNAs and 488 mRNAs were selected for further Ingenuity Pathway Analysis. Moreover, quantitative RT-PCR tests of most selected lncRNAs and mRNAs with high fold changes were consistent with the microarray data. We also performed ELISA to investigate the corresponding proteins levels of selected genes and showed that serum levels of SPP1, CD36, ATP6V0D2, CHI3L1, MYH11, and BDNF were differentially expressed in patients with coronary heart disease compared with healthy subjects. These proteins correlated with some biochemical parameters used in the diagnosis of cardiovascular diseases. Furthermore, receiver operating characteristic analysis showed a favorable diagnostic performance. The microarray profiling analysis and validation of differentially-expressed lncRNAs and mRNAs in atherosclerosis not only provide new insights into the pathogenesis of this disease but may also reveal new biomarkers for its diagnosis and treatment.

中文翻译：

---

新型长非编码RNA和mRNA作为潜在生物标志物及其在动脉粥样硬化中的功能的微阵列谱分析和验证。

长的非编码（lnc）RNA已被认为与动脉粥样硬化的发生和发展有关。然而，lncRNA在动脉粥样硬化中的表达及其作用机制仍不清楚。我们在人类晚期动脉粥样硬化斑块和正常动脉内膜中实施了微阵列分析，以检测lncRNA和mRNA表达谱。应用基因本体论功能丰富和途径分析来探讨动脉粥样硬化发病机理中潜在的功能和途径。总共选择了236个lncRNA和488个mRNA用于进一步的创造力途径分析。此外，大多数选择的具有高倍变化的lncRNA和mRNA的定量RT-PCR测试与微阵列数据一致。我们还进行了ELISA研究选定基因的相应蛋白质水平，并显示与健康受试者相比，冠心病患者的血清SPP1，CD36，ATP6V0D2，CHI3L1，MYH11和BDNF差异表达。这些蛋白质与用于诊断心血管疾病的一些生化参数相关。此外，接收机工作特性分析显示出良好的诊断性能。微阵列分析和动脉粥样硬化中差异表达的lncRNA和mRNA的验证不仅为这种疾病的发病机理提供了新的见解，而且还可能为诊断和治疗揭示新的生物标志物。与健康受试者相比，冠心病患者的BDNF和BDNF表达差异。这些蛋白质与用于诊断心血管疾病的一些生化参数相关。此外，接收机工作特性分析显示出良好的诊断性能。微阵列分析和动脉粥样硬化中差异表达的lncRNA和mRNA的验证不仅为这种疾病的发病机理提供了新的见解，而且还可能为诊断和治疗揭示新的生物标志物。与健康受试者相比，冠心病患者的BDNF和BDNF表达差异。这些蛋白质与用于诊断心血管疾病的一些生化参数相关。此外，接收机工作特性分析显示出良好的诊断性能。微阵列分析和动脉粥样硬化中差异表达的lncRNA和mRNA的验证不仅为这种疾病的发病机理提供了新的见解，而且还可能为诊断和治疗揭示新的生物标志物。