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Rainbowarray Microsphere-Based Gene Detection Assay.
SLAS Technology: Translating Life Sciences Innovation ( IF 2.5 ) Pub Date : 2019-11-02 , DOI: 10.1177/2472630319882319
Ying Xu 1 , Pei Zhang 1 , Fan Yi 1 , Jing Wei 2, 3 , Quan Du 1
Affiliation  

Here, we have developed a set of fluorophore-labeled microspheres named rainbowarray microspheres. Based on the spectrally encoded microspheres, we further developed a liquid hybridization approach for multiplex target detection. Different from the prototype Luminex xMAP array, this technology enables feasible, flexible, and cost-efficient microsphere labeling and multiplex detection in a timely and high-throughput manner. To demonstrate the practicability of this technology, quantitative measurement of microRNA regulation was performed during the differentiation of 3T3-L1 cells, in which the expression of two microRNAs was determined at a 2 h interval during a process of 2 days. The flexibility and the timely and high-throughput properties of the technology enable it to be widely implemented in clinical testing.

中文翻译:

基于Rainbowarray微球的基因检测测定。

在这里,我们开发了一组荧光团标记的微球,称为rainbowarray微球。基于光谱编码的微球,我们进一步开发了一种用于多重目标检测的液体杂交方法。与原型Luminex xMAP阵列不同,该技术可实现及时,高通量的可行,灵活,经济高效的微球标记和多重检测。为了证明该技术的实用性,在3T3-L1细胞分化过程中进行了microRNA调控的定量测量,其中在2天的过程中以2 h的间隔确定了两个microRNA的表达。该技术的灵活性以及及时和高通量的特性使它可以在临床测试中广泛实施。
更新日期:2020-04-10
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