Candida can adhere and form biofilm on biomaterials commonly used in medical devices which is a key attribute that enhances its ability to cause infections in humans. Furthermore, biomaterial-related infections represent a major therapeutic challenge since Candida biofilms are implicated in antifungal therapies failure. The goals of the present work were to investigate the effect of three 5-aminoimidazole-4-carbohydrazonamides, namely (Z)-5-amino-1-methyl-N′-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-fluorophenyl (1b), 3-fluorophenyl (1c)], on Candida albicans and Candida krusei biofilm on nanohydroxyapatite substrate, a well-known bioactive ceramic material. To address these goals, both quantitative methods (by cultivable cell numbers) and qualitative evaluation (by scanning electron microscopy) were used. Compounds cytocompatibility towards osteoblast-like cells was also evaluated after 24 h of exposure, through resazurin assay. The three tested compounds displayed a strong inhibitory effect on biofilm development of both Candida species as potent in vitro activity against C. albicans sessile cells. Regarding cytocompatibility, a concentration-dependent effect was observed. Together, these findings indicated that the potent activity of imidazole derivatives on Candida spp. biofilms on nanohydroxyapatite substrate, in particular compound 1c, is worth further investigating.

中文翻译：

---

5-Aminoimidazole-4-Carbohydrazonamides 衍生物对念珠菌的抑制作用。纳米羟基磷灰石基材上的生物膜

念珠菌可以在医疗器械中常用的生物材料上粘附并形成生物膜，这是增强其引起人类感染能力的关键属性。此外，生物材料相关的感染是一项重大的治疗挑战，因为念珠菌生物膜与抗真菌治疗失败有关。本工作的目标是研究三种 5-aminoimidazole-4-carbohydrazonamides，即 (Z)-5-amino-1-methyl-N'-aryl-1H-imidazole-4-carbohydrazonamides [aryl = phenyl (1a), 4-氟苯基 (1b), 3-氟苯基 (1c)]，在纳米羟基磷灰石基底上的白色念珠菌和克柔念珠菌生物膜上，纳米羟基磷灰石是一种众所周知的生物活性陶瓷材料。为了实现这些目标，使用了定量方法（通过可培养细胞数量）和定性评估（通过扫描电子显微镜）。在暴露 24 小时后，还通过刃天青测定评估了化合物对成骨细胞样细胞的细胞相容性。三种测试的化合物对两种念珠菌的生物膜发育显示出强烈的抑制作用，因为它具有针对白色念珠菌无蒂细胞的有效体外活性。关于细胞相容性，观察到浓度依赖性效应。总之，这些发现表明咪唑衍生物对念珠菌属的有效活性。纳米羟基磷灰石基底上的生物膜，特别是化合物 1c，值得进一步研究。关于细胞相容性，观察到浓度依赖性效应。总之，这些发现表明咪唑衍生物对念珠菌属的有效活性。纳米羟基磷灰石基底上的生物膜，特别是化合物 1c，值得进一步研究。关于细胞相容性，观察到浓度依赖性效应。总之，这些发现表明咪唑衍生物对念珠菌属的有效活性。纳米羟基磷灰石基底上的生物膜，特别是化合物 1c，值得进一步研究。