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Diversity of antimicrobial resistant pathogens from a freshwater ornamental fish farm
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2019-11-04 , DOI: 10.1111/lam.13231
P G Preena 1 , D Arathi 1 , N Sundar Raj 1 , T V Arun Kumar 1 , S Arun Raja 1 , R N Reshma 1 , T Raja Swaminathan 1
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The indiscriminate use of antimicrobials in aquaculture results in antibiotic selection pressure and proliferation of antimicrobial resistant (AMR) bacteria. Frequent assessment of antimicrobial resistance in aquaculture environment is inevitable so as to reduce the passage of clinically important AMR from aquatic to other environment. The present study analysed the antimicrobial resistance of pathogens associated with diseased koi carp and goldfish from an ornamental fish farm. Phenotypic and genotypic characterization of the recovered isolates from both fishes revealed significant pathogens in aquaculture such as Aeromonas, Edwardsiella tarda, Acinetobacter, Lactococcus, Citrobacter, Enterobacter and Comamonas. Shannon–Wiener diversity of koi isolates (2·359) was found to be higher than that of goldfish (1·864). Antibiotic susceptibility testing using disc diffusion with 47 antibiotics revealed significant resistance pattern of Acinetobacter, Comamonas, Klebsiella and Enterobacter from goldfish and Edwardsiella, Aeromonas, Lactococcus, Enterobacter and Acinetobacter from koi with higher multiple antibiotic resistance indexes (>0·3). The minimum inhibitory concentration of antibiotics for the major resistant isolates was found to be very high with >256 μg. All the isolates were susceptible to amoxicillin, kanamycin, cefepime, cefexime, cefotaxime, ceftazidime, doripenem, ciprofloxacin and norfloxacin, recommending their successful application in the farm.

中文翻译:

淡水观赏鱼养殖场抗微生物病原体的多样性

在水产养殖中不加区别地使用抗菌剂导致抗生素选择压力和抗菌素耐药性 (AMR) 细菌的增殖。在水产养殖环境中频繁评估抗菌素耐药性是不可避免的,以减少临床上重要的 AMR 从水生环境到其他环境的传播。本研究分析了与来自观赏鱼场的患病锦鲤和金鱼相关的病原体的抗菌素耐药性。从两种鱼类中回收的分离物的表型和基因型特征揭示了水产养殖中的重要病原体,如气单胞菌、迟发爱德华氏菌、不动杆菌、乳球菌、柠檬酸杆菌、肠杆菌和毛单胞菌。发现锦鲤分离株的香农-维纳多样性 (2·359) 高于金鱼 (1·864)。使用盘扩散法对47种抗生素进行的抗生素药敏试验显示,金鱼的不动杆菌、毛单胞菌、克雷伯菌和肠杆菌和锦鲤的爱德华氏菌、气单胞菌、乳球菌、肠杆菌和不动杆菌具有显着的耐药模式,多重耐药指数较高(>0·3)。发现主要耐药菌株的抗生素最低抑菌浓度非常高,>256 μg。所有分离株均对阿莫西林、卡那霉素、头孢吡肟、头孢克肟、头孢噻肟、头孢他啶、多利培南、环丙沙星和诺氟沙星敏感,推荐在养殖场成功应用。来自锦鲤的肠杆菌和不动杆菌具有较高的多重抗生素抗性指数(>0·3)。发现主要耐药菌株的抗生素最低抑菌浓度非常高,>256 μg。所有分离株均对阿莫西林、卡那霉素、头孢吡肟、头孢克肟、头孢噻肟、头孢他啶、多利培南、环丙沙星和诺氟沙星敏感,推荐在养殖场成功应用。来自锦鲤的肠杆菌和不动杆菌具有较高的多重抗生素抗性指数(>0·3)。发现主要耐药菌株的抗生素最低抑菌浓度非常高,>256 μg。所有分离株均对阿莫西林、卡那霉素、头孢吡肟、头孢克肟、头孢噻肟、头孢他啶、多利培南、环丙沙星和诺氟沙星敏感,推荐在养殖场成功应用。
更新日期:2019-11-04
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